Cisani G, Varaldo P E, Pompei R, Valisena S, Satta G
Istituto di Microbiologia, Università di Genova, Italy.
Microbios. 1989;59(239):73-83.
The formation of syncytia in cell monolayers infected with a macroplaque strain (MP) of herpes simplex virus was found to be inhibited by hen egg-white lysozyme. Inhibition was roughly proportional to the enzyme concentration. The virus titres in supernatant fluids of lysozyme-treated cultures were also reduced compared with untreated cultures. Control experiments excluded the possibility that lysozyme altered the virus viability and infectivity or impaired cell growth. Since lysozyme is a cationic protein, further experiments were performed in order to discover whether its antisyncytiogenic effect depended on its enzymatic activity or on its positive charge. Inhibition of the MP-induced polycaryocytosis was found to be caused by heat-inactivated lysozyme and three chemically-modified lysozymes with a higher positive charge (one retaining and two lacking enzymatic activity).
研究发现，感染单纯疱疹病毒大噬斑株（MP）的细胞单层中合胞体的形成受到鸡蛋清溶菌酶的抑制。抑制作用大致与酶浓度成正比。与未处理的培养物相比，溶菌酶处理的培养物上清液中的病毒滴度也有所降低。对照实验排除了溶菌酶改变病毒活力和感染性或损害细胞生长的可能性。由于溶菌酶是一种阳离子蛋白，因此进行了进一步的实验，以确定其抗合胞体形成作用是取决于其酶活性还是正电荷。结果发现，热灭活的溶菌酶和三种正电荷更高的化学修饰溶菌酶（一种保留酶活性，两种缺乏酶活性）可抑制MP诱导的多核细胞形成。
