Bardell D
Department of Biology, Kean College of New Jersey, Union 07083.
Microbios. 1989;59(239):93-100.
Droplets of tissue culture fluid containing herpes simplex virus type 1 were placed on the palm of the hand. Each 0.01 ml droplet was taken from a stock virus suspension with a titre of 10(7.5) TCID50/0.1 ml. At 0, 15, 30, 60 and 120 min a droplet was firmly touched with the middle finger of the right hand, after which, attempts were made to recover virus from the finger. At 0 min, when the virus-containing droplet was in a liquid state, there was a 100% rate of virus recovery. By 15 min the droplets had dried out, and after touching dried out droplets there was a 40% virus recovery rate, even though experimental procedures demonstrated that infectious virus was present in the dried out droplets at all test times. If the finger was moistened with tap water or saliva, there was a 100% recovery rate of virus after touching dried out droplets, as well as after touching droplets in a liquid state.
将含有1型单纯疱疹病毒的组织培养液滴置于手掌上。每0.01 ml液滴取自效价为10(7.5) TCID50/0.1 ml的病毒储备悬液。在0、15、30、60和120分钟时，用右手食指轻轻触碰一个液滴，之后尝试从手指上回收病毒。在0分钟时，当含病毒液滴呈液态时，病毒回收率为100%。到15分钟时，液滴已干涸，触碰干涸液滴后病毒回收率为40%，尽管实验程序表明在所有测试时间干涸液滴中均存在感染性病毒。如果用自来水或唾液湿润手指，触碰干涸液滴以及触碰液态液滴后病毒回收率均为100%。
