Graham M L, Springthorpe V S, Sattar S A
Department of Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ontario, Canada.
Appl Environ Microbiol. 1996 Nov;62(11):4252-5. doi: 10.1128/aem.62.11.4252-4255.1996.
We report an ex vivo method, which uses pieces of human skin excised during routine plastic surgery, for testing survival of hazardous pathogens. Using this procedure, we compared the survival of human herpesvirus 2 on human skin and on metal disks. At the physiological skin temperature of 32 degrees C, the half-life of the virus on skin was 1.44 h while on metal disks it was 0.36 h. Even at ambient temperature (22 degrees C), the virus lost infectivity faster (half-life = 0.96 h) on metal disks than on the skin at 32 degrees C. The method described could be used to assess the survival of other human pathogens on skin and to evaluate the germicidal activity of handwashing agents and other topicals.
我们报告了一种体外方法，该方法使用在常规整形手术中切除的人体皮肤碎片来测试有害病原体的存活情况。通过这个程序，我们比较了人疱疹病毒2在人体皮肤上和金属盘上的存活情况。在皮肤生理温度32摄氏度时，该病毒在皮肤上的半衰期为1.44小时，而在金属盘上为0.36小时。即使在环境温度（22摄氏度）下，该病毒在金属盘上丧失感染力的速度（半衰期 = 0.96小时）也比在32摄氏度的皮肤上更快。所描述的方法可用于评估其他人类病原体在皮肤上的存活情况，并评估洗手剂和其他局部用药的杀菌活性。
