Heggie P, Burdon T, Lowe J, Landon M, Lennox G, Jefferson D, Mayer R J
Department of Biochemistry, Nottingham University Medical School, Queens Medical Centre, U.K.
Neurosci Lett. 1989 Jul 31;102(2-3):343-8. doi: 10.1016/0304-3940(89)90103-1.
A restriction fragment of the coding region of a human ubiquitin gene has been used in Northern analyses of RNA prepared from human motor cortex and anterior horn region of cervical spinal cord. The analyses show that there is a substantial increase (approximately two-fold) in the expression of a polyubiquitin gene in motor cortex and spinal cord from patients with motor neurone disease compared to these tissues from control cases. Polyubiquitin gene expression in other organisms is associated with physical or chemical cell stresses. The data indicate that the primary stresses which result in the generation of ubiquitinated filamentous inclusion bodies in neurones in motor neurone disease also result in increased transcription of a gene coding for a polyprotein of ubiquitin.
人类泛素基因编码区的一个限制性片段已被用于对取自人类运动皮层和颈髓前角区域的RNA进行Northern分析。分析表明，与对照病例的这些组织相比，运动神经元疾病患者的运动皮层和脊髓中多聚泛素基因的表达有显著增加（约两倍）。其他生物体中的多聚泛素基因表达与物理或化学细胞应激相关。数据表明，导致运动神经元疾病中神经元产生泛素化丝状包涵体的主要应激也导致编码泛素多蛋白的基因转录增加。
