Rajendran Kumar, Karunagaran Vithiya, Mahanty Biswanath, Sen Shampa
Industrial Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore 632 014, Tamil Nadu, India.
Department of Environmental Engineering, INHA University, Incheon, Republic of Korea.
Int J Biol Macromol. 2015 Mar;74:376-81. doi: 10.1016/j.ijbiomac.2014.12.028. Epub 2014 Dec 24.
Iron oxide nanoparticles were gaining significant importance in a variety of applications due to its paramagnetic properties and biocompatibility. Various chemical methods were employed for hematite nanoparticle synthesis which require special equipment or a complex production process. In this study, protein capped crystalline hexagonal hematite (α-Fe2O3) nanoparticles were synthesized by green approach using culture supernatant of a newly isolated bacterium, Bacillus cereus SVK1 at ambient conditions. The synthesized nanoparticles were characterized by electron microscopy, X-ray diffraction, UV-visible spectroscopy and Fourier transform infrared spectroscopic analysis. Nanoparticles were evaluated for its possible anticancer activity against HepG2 liver cancer cells by MTT assay. Hematite nanoparticles with an average diameter of 30.2 nm, exhibited a significant cytotoxicity toward HepG2 cells in a concentration-dependent manner (CTC50=704 ng/ml).
由于其顺磁性和生物相容性，氧化铁纳米颗粒在各种应用中变得越来越重要。采用了各种化学方法来合成赤铁矿纳米颗粒，这些方法需要特殊设备或复杂的生产工艺。在本研究中，在环境条件下，使用新分离的蜡状芽孢杆菌SVK1的培养上清液，通过绿色方法合成了蛋白质包覆的结晶六方赤铁矿（α-Fe2O3）纳米颗粒。通过电子显微镜、X射线衍射、紫外可见光谱和傅里叶变换红外光谱分析对合成的纳米颗粒进行了表征。通过MTT法评估纳米颗粒对HepG2肝癌细胞的可能抗癌活性。平均直径为30.2 nm的赤铁矿纳米颗粒对HepG2细胞表现出显著的细胞毒性，且呈浓度依赖性（半数细胞毒性浓度=704 ng/ml）。
