Department of Plant Sciences, Quaid-I-Azam University, Islamabad, 45320, Pakistan.
Department of Botany, Bacha Khan University, Charsadda, Khyber Pakhtunkhwa, Pakistan.
Sci Rep. 2021 Oct 25;11(1):20988. doi: 10.1038/s41598-021-99839-z.
Due to their versatile applications, ZnONPs have been formulated by several approaches, including green chemistry methods. In the current study, convenient and economically viable ZnONPs were produced using Elaeagnus angustifolia (EA) leaf extracts. The phytochemicals from E. angustifolia L. are believed to serve as a non-toxic source of reducing and stabilizing agents. The physical and chemical properties of ZnONPs were investigated employing varying analytical techniques (UV, XRD, FT-IR, EDX, SEM, TEM, DLS and Raman). Strong UV-Vis absorption at 399 nm was observed for green ZnONPs. TEM, SEM and XRD analyses determined the nanoscale size, morphology and crystalline structure of ZnONPs, respectively. The ZnONPs were substantiated by evaluation using HepG2 (IC: 21.7 µg mL) and HUH7 (IC: 29.8 µg mL) cancer cell lines and displayed potential anticancer activities. The MTT cytotoxicity assay was conducted using Leishmania tropica "KWH23" (promastigotes: IC, 24.9 µg mL; and amastigotes: IC, 32.83 µg mL). ZnONPs exhibited excellent antimicrobial potencies against five different bacterial and fungal species via the disc-diffusion method, and their MIC values were calculated. ZnONPs were found to be biocompatible using human erythrocytes and macrophages. Free radical scavenging tests revealed excellent antioxidant activities. Enzyme inhibition assays were performed and revealed excellent potential. These findings suggested that EA@ZnONPs have potential applications and could be used as a promising candidate for clinical development.
由于其多功能的应用，ZnONPs 已经通过几种方法进行了配方，包括绿色化学方法。在本研究中，使用沙枣（Elaeagnus angustifolia，EA）叶提取物方便且经济地制备了 ZnONPs。沙枣中的植物化学物质被认为是无毒的还原剂和稳定剂来源。通过使用不同的分析技术（UV、XRD、FT-IR、EDX、SEM、TEM、DLS 和 Raman）研究了 ZnONPs 的物理和化学性质。观察到绿色 ZnONPs 在 399nm 处有强的紫外-可见吸收。TEM、SEM 和 XRD 分析分别确定了 ZnONPs 的纳米尺寸、形态和晶体结构。通过 HepG2（IC：21.7µgmL）和 HUH7（IC：29.8µgmL）癌细胞系的评估证实了 ZnONPs，并显示出潜在的抗癌活性。使用 Leishmania tropica“KWH23”（前鞭毛体：IC，24.9µgmL；和无鞭毛体：IC，32.83µgmL）进行了 MTT 细胞毒性测定。通过圆盘扩散法测定 ZnONPs 对五种不同细菌和真菌的抗菌活性，计算 MIC 值。ZnONPs 在人红细胞和巨噬细胞中表现出良好的生物相容性。自由基清除试验显示出良好的抗氧化活性。进行了酶抑制试验，显示出良好的潜力。这些发现表明，EA@ZnONPs 具有潜在的应用价值，可作为临床开发的有前途的候选物。
