[Resveratrol improves intestinal injury in hemorrhagic shock rats by protection of mitochondria and reduction of oxidative stress].

OBJECTIVE

To explore whether resveratrol can reduce intestinal damage in hemorrhagic shock rats and the underlying mechanism.

METHODS

A total of 24 Sprague-Dawley rats of specifi c pathogen free (SPF) were randomly divided into a control group(n=8), a resveratrol group (SR group, n=8) and a vehicle group (SS group, n=8). Th e mean arterial pressure was recorded. Two hours aft er hemorrhagic shock, 15 mg/kg resveratrol or 0.3 mL equal volume of vehicle and autologous blood were given, respectively. The intestinal specimens were collected for hematoxylin-eosin (HE) staining and calculated the pathological score. The superoxide dismutase 2 (SOD2) and cytochrome C (Cyt C) protein expression was examined by immunohistochemistry and/or Western blot. ATP level, activities of glutathione peroxidase (GXH-px), catalase (CAT) and SOD were also detected.

RESULTS

Two hours after autologous blood transfusion, the mean arterial pressure in the SR group was significantly higher than that in the SS group (P< 0.01). Compared with the SS group, the pathological injury was significantly alleviated and pathological scores were dramatically reduced in the SR group (P< 0.05). The activities of GXH-px, CAT, SOD and the ATP levels in the SR group were significantly higher than those in the SS group (all P< 0.01). Compared with the SS group, the SOD2 expression was significantly higher while the Cyt C expression was dramatically lower in the SR group (both P< 0.01).

CONCLUSION

Resveratrol could alleviate the intestinal injury in hemorrhagic shock rats, which might be associated with its effects on reduction of oxidative stress and protection of mitochondria.