Gokul Raj K, Manikandan R, Arulvasu C, Pandi M
Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai 625 021, Tamil Nadu, India.
Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Mar 5;138:667-74. doi: 10.1016/j.saa.2014.11.036. Epub 2014 Dec 9.
Cladosporium oxysporum a new taxol producing endophytic fungus was identified and production of taxol were characterized using UV-visible spectroscopy (UV-vis), high-performance liquid chromatography (HPLC), infrared (IR) nuclear magnetic resonance spectroscopy (NMR ((13)C and (1)H)) and liquid chromatography-mass spectrometry (LC-MS). The taxol biosynthetic gene (dbat) was evaluated for new taxol producing fungus. Antibacterial activity against six different human pathogenic bacteria was done by agar well diffusion method. The anticancer efficacy of isolated fungal taxol were also evaluated in human colon cancer cell HCT 15 by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), cytotoxicity and nuclear morphology analysis. The isolated fungal taxol showed positive towards biosynthetic gene (dbat) and effective against both Gram positive as well as Gram negative. The fungal taxol suppress growth of cancer cell line HCT 15 with an IC50 value of 3.5μM concentration by 24h treatment. Thus, the result reveals that C. oxysporum could be a potential alternative source for production of taxol and have antibacterial as well as anticancer properties with possible clinical applications.
尖孢枝孢菌是一种新发现的产紫杉醇内生真菌，通过紫外可见光谱（UV-vis）、高效液相色谱（HPLC）、红外光谱（IR）、核磁共振光谱（NMR（¹³C和¹H））和液相色谱-质谱联用（LC-MS）对其紫杉醇的产生进行了表征。对新的产紫杉醇真菌评估了紫杉醇生物合成基因（dbat）。采用琼脂孔扩散法对六种不同的人类致病细菌进行了抗菌活性测试。还通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）、细胞毒性和核形态分析，在人结肠癌细胞HCT 15中评估了分离得到的真菌紫杉醇的抗癌功效。分离得到的真菌紫杉醇对生物合成基因（dbat）呈阳性，对革兰氏阳性菌和革兰氏阴性菌均有效。经24小时处理，真菌紫杉醇以3.5μM的浓度抑制癌细胞系HCT 15的生长，IC50值为该浓度。因此，结果表明尖孢枝孢菌可能是生产紫杉醇的潜在替代来源，具有抗菌和抗癌特性，可能具有临床应用价值。
