Wang Zhiqiang, Cai Ming, Weng Yuan, Zhang Fang, Meng Dong, Song Jun, Zhou Huan, Xie Zongtao
Department of Thoracic and Cardiovascular Surgery, Affiliated Hospital of Jiangnan University, The Forth People's Hospital of Wuxi City, No. 200, Huihe Road, Wuxi, 214062, China.
J Cancer Res Clin Oncol. 2015 Aug;141(8):1353-61. doi: 10.1007/s00432-014-1903-0. Epub 2014 Dec 28.
Metastasis-associated in colon cancer-1 (MACC1) is a newly identified gene that plays an important role in cancer progression and metastasis. MACC1 has important functions in the differentiation, invasion, and metastasis of nonsmall cell lung cancer (NSCLC). However, the value of circulating MACC1 as a potential diagnostic and prognostic biomarker for NSCLC remains unknown.
Plasma MACC1 mRNA levels were examined in 272 patients with NSCLC, 61 with benign lung disease, and 80 healthy volunteers using reverse transcription quantitative real-time polymerase chain reaction.
MACC1 was more highly expressed in NSCLC patients than in patients with benign disease (P < 0.001) or in healthy volunteers (P < 0.001). High MACC1 expression was significantly associated with NSCLC stage (P = 0.013) and lymph node metastasis (P = 0.016). The area under the receiver operating characteristic curve was 0.766, and the optimal cutoff value was 0.105, providing a sensitivity of 71.4 % and a specificity of 89.1 %. The diagnostic capability of circulating MACC1 mRNA was higher than that of carcinoembryonic antigen (P = 0.025) or cytokeratin-19 (P = 0.010). Furthermore, high MACC1 expression was associated with poor overall survival (OS) and disease-free survival (DFS) and predicted poor survival in NSCLC patients. Consequently, MACC1 mRNA was an independent prognostic factor of OS and DFS.
We concluded that circulating MACC1 mRNA represents a potential noninvasive, diagnostic and prognostic marker for NSCLC.
结肠癌转移相关基因1（MACC1）是一个新发现的基因，在癌症进展和转移中起重要作用。MACC1在非小细胞肺癌（NSCLC）的分化、侵袭和转移中具有重要功能。然而，循环MACC1作为NSCLC潜在诊断和预后生物标志物的价值仍不清楚。
采用逆转录定量实时聚合酶链反应检测272例NSCLC患者、61例良性肺病患者和80名健康志愿者血浆中MACC1 mRNA水平。
MACC1在NSCLC患者中的表达高于良性疾病患者（P < 0.001）或健康志愿者（P < 0.001）。MACC1高表达与NSCLC分期（P = 0.013）和淋巴结转移（P = 0.016）显著相关。受试者工作特征曲线下面积为0.766，最佳截断值为0.105，敏感性为71.4%，特异性为89.1%。循环MACC1 mRNA的诊断能力高于癌胚抗原（P = 0.025）或细胞角蛋白19（P = 0.010）。此外，MACC1高表达与总生存期（OS）和无病生存期（DFS）较差相关，并预测NSCLC患者生存不良。因此，MACC1 mRNA是OS和DFS的独立预后因素。
我们得出结论，循环MACC1 mRNA是NSCLC一种潜在的非侵入性诊断和预后标志物。
