Pan Chieh-Yu, Lin Chao-Nan, Chiou Ming-Tang, Yu Chao Yuan, Chen Jyh-Yih, Chien Chi-Hsien
Department and Graduate Institute of Aquaculture, National Kaohsiung Marine University, Nanzih Dist., Kaohsiung, Taiwan.
Graduate Institute and Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan.
Oncotarget. 2015 Feb 10;6(4):2290-301. doi: 10.18632/oncotarget.2959.
Pardaxin is an antimicrobial peptide of 33 amino acids, originally isolated from marine fish. We previously demonstrated that pardaxin has anti-tumor activity against murine fibrosarcoma, both in vitro and in vivo. In this study, we examined the anti-tumor activity, toxicity profile, and maximally-tolerated dose of pardaxin treatment in dogs with different types of refractory tumor. Local injection of pardaxin resulted in a significant reduction of perianal gland adenoma growth between 28 and 38 days post-treatment. Surgical resection of canine histiocytomas revealed large areas of ulceration, suggesting that pardaxin acts like a lytic peptide. Pardaxin treatment was not associated with significant variations in blood biochemical parameters or secretion of immune-related proteins. Our findings indicate that pardaxin has strong therapeutic potential for treating perianal gland adenomas in dogs. These data justify the veterinary application of pardaxin, and also provide invaluable information for veterinary medicine and future human clinical trials.
豹鳎毒素是一种由33个氨基酸组成的抗菌肽，最初从海鱼中分离出来。我们之前证明，豹鳎毒素在体外和体内均对小鼠纤维肉瘤具有抗肿瘤活性。在本研究中，我们检测了豹鳎毒素对患有不同类型难治性肿瘤的犬的抗肿瘤活性、毒性特征和最大耐受剂量。局部注射豹鳎毒素导致治疗后28至38天肛周腺腺瘤生长显著减少。犬组织细胞瘤的手术切除显示有大面积溃疡，这表明豹鳎毒素的作用类似于溶解肽。豹鳎毒素治疗与血液生化参数或免疫相关蛋白分泌的显著变化无关。我们的研究结果表明，豹鳎毒素在治疗犬肛周腺腺瘤方面具有强大的治疗潜力。这些数据证明了豹鳎毒素在兽医领域的应用合理性，也为兽医学和未来的人类临床试验提供了宝贵信息。
