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林麝(Moschus berezovskii)不同类型分泌物中麝香成分的研究。

Study of compositions of musks in different types secreted by forest musk deer (Moschus berezovskii).

机构信息

School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, People's Republic of China.

出版信息

PLoS One. 2021 Mar 16;16(3):e0245677. doi: 10.1371/journal.pone.0245677. eCollection 2021.

Abstract

Musk is a secretion of the forest musk deer (Moschus berezovskii). Normal musk is a brown solid secretion with a light fragrance. In this study, abnormal types of musk, namely, white and black musks, were discovered during the musk collection process. Researchers have long been concerned with the components of musk. Herein, GC-MS, headspace solid-phase microextraction (HS-SPME), and nonmetric multidimensional scaling (NMDS) were used to analyze the nonpolar organic components, volatile organic components, and sample similarities among different musks, respectively. Abundant steroid hormones and proteins were also found in the musk. The steroid hormone concentrations were detected using a radioimmunoassay (RIA). Proteins in the samples were hydrolyzed and the amino acids concentrations were detected. The steroid hormone and amino acid concentrations in white musk were significantly lower than in normal and black musks (p<0.05). The components were subjected to NMDS analysis to understand the differences in components among different types of musk, with the results suggesting that white musk was different from normal and black musks.

摘要

麝香是林麝(Moschus berezovskii)的分泌物。正常麝香是一种棕色的固体分泌物,具有淡淡的香气。在这项研究中,在麝香采集过程中发现了异常类型的麝香,即白麝香和黑麝香。研究人员长期以来一直关注麝香的成分。在此,分别使用气相色谱-质谱联用(GC-MS)、顶空固相微萃取(HS-SPME)和非度量多维尺度分析(NMDS)来分析非极性有机成分、挥发性有机成分和不同麝香之间的样品相似性。麝香中还发现了丰富的甾体激素和蛋白质。使用放射免疫分析(RIA)检测甾体激素浓度。对样品中的蛋白质进行水解,并检测氨基酸浓度。白麝香中的甾体激素和氨基酸浓度明显低于正常麝香和黑麝香(p<0.05)。对成分进行 NMDS 分析,以了解不同类型麝香之间成分的差异,结果表明白麝香与正常麝香和黑麝香不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70bf/7963063/665a69f02c18/pone.0245677.g001.jpg

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