Van Fraga Iva Tereza, Limborço-Filho Marcelo, Lima Onésia Cristina Oliveira, Lacerda-Queiroz Norinne, Guidine Patrícia Alves Maia, Moraes Márcio Flávio Dutra, Nascimento Araújo Ricardo, Moraes-Santos Tasso, Massensini André Ricardo, Arantes Rosa Maria Esteves, Carvalho-Tavares Juliana
Núcleo de Neurociências (NNC), Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), MG, Brazil.
Núcleo de Neurociências (NNC), Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), MG, Brazil; Centro de Tecnologia e Imagem em Magneto-Ressonância (CTPMag), Escola de Engenharia, UFMG, Brazil.
Neurosci Lett. 2015 Feb 19;588:24-8. doi: 10.1016/j.neulet.2014.12.044. Epub 2014 Dec 26.
Accidents caused by scorpion stings, mainly affecting children, are considered an important cause of morbidity and mortality in tropical countries. Clinical studies demonstrate the relevant role of systemic inflammatory events in scorpion envenoming. However, remains poorly understood whether the major lethal component in Tityus serrulatus venom, tityustoxin (TsTX), is able to induce inflammatory responses in the cerebral microcirculation. In this study, we systematically examined leukocyte recruitment into the CNS in response to TsTX injection. Accordingly, developing rats were subjected to a subcutaneous (s.c.) injection of TsTX (0.75mg/kg), and leukocyte recruitment (i.e., 4, 8 and 12h after injection) and TNF-α levels were evaluated. Rats injected with TsTX presented a significant increase in leukocyte rolling and adhesion and higher levels of TNF-α at all time points studied, compared to the control group. Altogether, this work demonstrates the triggering of neuroimmunological mechanisms induced by TsTX injection in young rats.
蝎子蜇伤导致的事故主要影响儿童，在热带国家被认为是发病和死亡的重要原因。临床研究表明全身炎症事件在蝎子蜇伤中毒中起相关作用。然而，对于锯齿脂鲤属蝎子毒液中的主要致死成分——锯齿脂鲤毒素（TsTX）是否能够在脑微循环中诱导炎症反应，仍知之甚少。在本研究中，我们系统地检测了注射TsTX后白细胞向中枢神经系统的募集情况。相应地，对发育中的大鼠进行皮下注射TsTX（0.75mg/kg），并评估白细胞募集情况（即注射后4、8和12小时）以及TNF-α水平。与对照组相比，注射TsTX的大鼠在所有研究时间点的白细胞滚动和黏附均显著增加，且TNF-α水平更高。总之，这项工作证明了注射TsTX可在幼鼠中触发神经免疫机制。
