Astapenko D, Jor O, Lehmann C, Cerny V
Department of Anesthesiology and Intensive Care Medicine, Faculty of Medicine Hradec Kralove, University Hospital Hradec Kralove, Charles University in Prague, Czech Republic.
J Microsc. 2015 Feb;257(2):161-5. doi: 10.1111/jmi.12198. Epub 2014 Dec 24.
For microcirculation research there is a need for baseline data and feasibility protocols describing microcirculation of various organs. The aim of our study was to examine the reliability and reproducibility of sidestream dark-field (SDF) imaging within the renal cortical microcirculation in rats. Renal microcirculation was observed using SDF probe placed on the exposed renal surface via the upper midline laparotomy. Video sequences recorded intermittently in short apneic pauses were analyzed off-line by using AVA 3.0 software (MicroVision Medical, Amsterdam, the Netherlands). Results are expressed as mean (SD) or median (25-75% percentiles). We obtained 60 clear sequences from all recorded analyzable videos from all the animals. The total small vessel and all vessel density (in mm.mm(-2) ) were (28.79 ± 0.40) and (28.95 ± 0.40), respectively. The perfused small and all vessel density were (28.79 ± 0.40) and (28.95 ± 0.40), respectively. The DeBacker Score was (19.14 ± 0.43), the proportion of perfused vessels was 100% (100-100%) and the microvascular flow index was 3.49 (3-3.75). We conclude SDF imaging provides a reliable method to examine the renal microvascular bed in vivo and thus can be used for the study of the renal cortical vascular network in various experimental diseases models and clinical settings.
对于微循环研究，需要有关各种器官微循环的基线数据和可行性方案。我们研究的目的是检验大鼠肾皮质微循环中侧流暗场（SDF）成像的可靠性和可重复性。通过上腹部中线剖腹术将SDF探头置于暴露的肾表面，观察肾微循环。使用AVA 3.0软件（荷兰阿姆斯特丹MicroVision Medical公司）对在短暂呼吸暂停期间间歇性记录的视频序列进行离线分析。结果以平均值（标准差）或中位数（第25 - 75百分位数）表示。我们从所有动物记录的可分析视频中获得了60个清晰的序列。小血管总密度和所有血管密度（单位：mm.mm(-2)）分别为(28.79 ± 0.40)和(28.95 ± 0.40)。灌注的小血管密度和所有血管密度分别为(28.79 ± 0.40)和(28.95 ± 0.40)。德贝克尔评分是(19.14 ± 0.43)，灌注血管比例为100%（100 - 100%），微血管血流指数为3.49（3 - 3.75）。我们得出结论，SDF成像为体内检查肾微血管床提供了一种可靠的方法，因此可用于各种实验性疾病模型和临床环境中肾皮质血管网络的研究。
