Graupp Matthias, Gruber Hans-Jürgen, Weiss Gregor, Kiesler Karl, Bachna-Rotter Sophie, Friedrich Gerhard, Gugatschka Markus
ENT University Hospital Graz, Department of Phoniatrics, Medical University Graz, Graz, Austria.
Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University Graz, Graz, Austria.
Laryngoscope. 2015 Jun;125(6):E203-9. doi: 10.1002/lary.25103. Epub 2014 Dec 29.
OBJECTIVES/HYPOTHESIS: Vocal fold fibrosis represents a major disease burden. Screening of antifibrotic compounds could be facilitated by an in vitro fibrogenesis system. Limitations of existing models might be overcome by implication of the excluded volume effect.
In-vitro study.
Vocal fold fibroblasts obtained from rats' lamina propria were cultured in four different settings: in standard medium, under "crowded" conditions by adding inert macromolecules, under external administration of transforming growth factor (TGF)ß-1, and under a combination of both. After 5 days, supernatant and cell layer were collected and analyzed by enzyme-linked immunosorbent assay. Immunofluorescence was additionally performed.
Collagen-alpha1(I) deposition increased significantly under crowded conditions and after administration of TGFβ-1. Amounts of collagen in the cell layer were significantly higher under crowding conditions with TGFβ-1 compared to administration of TGFβ-1 alone.
Crowding enhanced collagen deposition, resulting in more favorable conditions for studying fibrogenesis. This can be the first step toward developing a robust in vitro model for testing antifibrotic compounds.
NA.
目的/假设：声带纤维化是一项主要的疾病负担。体外纤维化生成系统有助于抗纤维化化合物的筛选。现有模型的局限性可能通过引入排阻体积效应来克服。
体外研究。
从大鼠固有层获取的声带成纤维细胞在四种不同条件下培养：在标准培养基中、通过添加惰性大分子在“拥挤”条件下、在外部给予转化生长因子（TGF）β-1以及在两者结合的条件下。5天后，收集上清液和细胞层，并通过酶联免疫吸附测定法进行分析。另外进行了免疫荧光检测。
在拥挤条件下以及给予TGFβ-1后，胶原蛋白α1（I）沉积显著增加。与单独给予TGFβ-1相比，在拥挤条件下联合给予TGFβ-1时，细胞层中的胶原蛋白量显著更高。
拥挤增强了胶原蛋白沉积，为研究纤维化生成创造了更有利的条件。这可能是开发用于测试抗纤维化化合物的强大体外模型的第一步。
无。
