Voytek P, Kozak C
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Virology. 1988 Aug;165(2):469-75. doi: 10.1016/0042-6822(88)90590-9.
We isolated a novel infectious murine leukemia virus (HoMuLV) from the wild mouse Mus hortulanus. HoMuLV has an ecotropic virus host range, but the viral DNA fails to hybridize to viral envelope segments specific for the known inbred and wild mouse ecotropic as well as nonecotropic MuLVs. Despite this difference in its env gene, HoMuLV appears to use the same ecotropic cell-surface receptor since it infects only hamster and mouse somatic cell hybrids which contain the Rec-1 ecotropic virus receptor on chromosome 5. Furthermore, HoMuLV does not infect mice carrying the Fv-4r allele which is thought to prevent ecotropic virus infection through an interference mechanism. HoMuLV is NB-tropic and, unlike other infectious MuLVs, does not grow in cells derived from the wild mouse species. M. dunni. Five to ten months after neonatal inoculation with HoMuLV, 72% of female NIH Swiss mice (8/11) contracted lymphoma or erythroid leukemia, but 33% of the inoculated males (5/15) developed erythroid or myelogenous leukemia within 8-16 months. These data suggest that NIH Swiss males and females differ in their susceptibility to HoMuLV-induced disease. Furthermore, NIH Swiss mice were found to be more susceptible to HoMuLV-induced disease than NFS/N mice. Tumors contained infectious MCF virus, which is consistent with the hypothesis that MCF virus may mediate tumorigenesis by HoMuLV.
我们从野生小鼠短尾鼩鼱（Mus hortulanus）中分离出一种新型传染性小鼠白血病病毒（HoMuLV）。HoMuLV具有亲嗜性病毒宿主范围，但病毒DNA无法与已知近交系和野生小鼠亲嗜性以及非亲嗜性MuLVs的病毒包膜片段杂交。尽管其env基因存在这种差异，但HoMuLV似乎使用相同的亲嗜性细胞表面受体，因为它仅感染在5号染色体上含有Rec-1亲嗜性病毒受体的仓鼠和小鼠体细胞杂种。此外，HoMuLV不感染携带Fv-4r等位基因的小鼠，该等位基因被认为通过干扰机制阻止亲嗜性病毒感染。HoMuLV是NB嗜性的，与其他传染性MuLVs不同，它不在源自野生小鼠物种短尾小鼠（M. dunni）的细胞中生长。用HoMuLV对新生小鼠进行接种后5至10个月，72%的雌性NIH瑞士小鼠（8/11）患上淋巴瘤或红白血病，但33%的接种雄性小鼠（5/15）在8至16个月内发生红白血病或髓性白血病。这些数据表明，NIH瑞士雄性和雌性小鼠对HoMuLV诱导疾病的易感性存在差异。此外，发现NIH瑞士小鼠比NFS/N小鼠对HoMuLV诱导的疾病更易感。肿瘤中含有传染性MCF病毒，这与MCF病毒可能介导HoMuLV致瘤的假说一致。
