Kumari A, Papenfus H B, Kulkarni M G, Pošta M, Van Staden J
Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Scottsville, South Africa.
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Plant Biol (Stuttg). 2015 Jul;17(4):825-30. doi: 10.1111/plb.12300. Epub 2015 Jan 23.
Plant-derived smoke stimulates seed germination in numerous plant species. Smoke also has a positive stimulatory effect on pollen germination and pollen tube growth. The range of plant families affected my smoke still needs to be established since the initial study was restricted to only three species from the Amaryllidaceae. The effects of smoke-water (SW) and the smoke-derived compounds, karrikinolide (KAR1 ) and trimethylbutenolide (TMB) on pollen growth characteristics were evaluated in seven different plant families. Smoke-water (1:1000 and 1:2000 v:v) combined with either Brewbaker and Kwack's (BWK) medium or sucrose and boric acid (SB) medium significantly improved pollen germination and pollen tube growth in Aloe maculata All., Kniphofia uvaria Oken, Lachenalia aloides (L.f.) Engl. var. aloides and Tulbaghia simmleri P. Beauv. Karrikinolide (10(-6) and 10(-7) m) treatment significantly improved pollen tube growth in A. maculata, K. uvaria, L. aloides and Nematanthus crassifolius (Schott) Wiehle compared to the controls. BWK or SB medium containing TMB (10(-3) m) produced significantly longer pollen tubes in A. maculata, K. uvaria and N. crassifolius. These results indicate that plant-derived smoke and the smoke-isolated compounds may stimulate pollen growth in a wide range of plant species.
植物源烟雾能刺激众多植物物种的种子萌发。烟雾对花粉萌发和花粉管生长也有积极的促进作用。由于最初的研究仅局限于石蒜科的三个物种，因此受烟雾影响的植物科的范围仍有待确定。我们评估了烟雾水（SW）以及烟雾衍生化合物卡里金内酯（KAR1）和三甲基丁烯内酯（TMB）对七个不同植物科花粉生长特性的影响。在斑纹芦荟、火炬花、绵枣儿、紫娇花中，1:1000和1:2000（体积比）的烟雾水与布鲁贝克和夸克（BWK）培养基或蔗糖和硼酸（SB）培养基组合，显著提高了花粉萌发率和花粉管生长速度。与对照相比，10⁻⁶和10⁻⁷mol/L的卡里金内酯处理显著促进了斑纹芦荟、火炬花、绵枣儿和粗叶纽扣苣苔的花粉管生长。在斑纹芦荟、火炬花和粗叶纽扣苣苔中，含10⁻³mol/L三甲基丁烯内酯的BWK或SB培养基能使花粉管长得更长。这些结果表明，植物源烟雾和从烟雾中分离出的化合物可能会刺激多种植物物种的花粉生长。
