Barbeck Mike, Lorenz Jonas, Kubesch Alica, Böhm Nicole, Booms Patrick, Choukroun Joseph, Sader Robert, Kirkpatrick Charles James, Ghanaati Shahram
1 Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt am Main, Germany.
2 Pain Therapy Center, Nice, France.
J Oral Implantol. 2015 Dec;41(6):e238-51. doi: 10.1563/aaid-joi-D-14-00274. Epub 2014 Dec 29.
In this study, the tissue reactions to 2 new porcine dermis-derived collagen membranes of different thickness were analyzed. The thicker material (Mucoderm) contained sporadically preexisting vessel skeletons and fatty islands. The thinner membrane (Collprotect) had a bilayered structure (porous and occlusive side) without any preexisting structures. These materials were implanted subcutaneously in mice to analyze the tissue reactions and potential transmembranous vascularization. Histological and histomorphometrical methodologies were performed at 4 time points (3, 10, 15, and 30 days). Both materials permitted stepwise connective tissue ingrowth into their central regions. In the Mucoderm matrix, newly built microvessels were found within the preexisting vessel and fatty island skeletons after 30 days. This vascularization was independent of the inflammation-related vascularization on both material surfaces. The Collprotect membrane underwent material disintegration by connective tissue strands in combination with vessels and multinucleated giant cells. The histomorphometric analyses revealed that the thickness of Mucoderm did not decrease significantly, while an initial significant decrease of membrane thickness in the case of Collprotect was found at day 15. The present results demonstrate that the 2 analyzed collagen membranes underwent a multinucleated giant cell-associated vascularization. Neither of the materials underwent transmembraneous vascularization. The microvessels were found within the preexisting vessel and fatty island skeletons. Additional long-term studies and clinical studies are necessary to determine how the observed foreign body giant cells affect tissue regeneration.
在本研究中，分析了两种不同厚度的新型猪真皮来源胶原膜的组织反应。较厚的材料（Mucoderm）偶尔含有预先存在的血管骨架和脂肪岛。较薄的膜（Collprotect）具有双层结构（多孔侧和封闭侧），没有任何预先存在的结构。将这些材料皮下植入小鼠体内，以分析组织反应和潜在的跨膜血管化。在4个时间点（3天、10天、15天和30天）进行了组织学和组织形态计量学方法研究。两种材料都允许结缔组织逐步向内生长至其中心区域。在Mucoderm基质中，30天后在预先存在的血管和脂肪岛骨架内发现了新形成的微血管。这种血管化独立于两种材料表面与炎症相关的血管化。Collprotect膜通过结缔组织束与血管和多核巨细胞共同作用而发生材料崩解。组织形态计量学分析显示，Mucoderm的厚度没有显著降低，而Collprotect在第15天时膜厚度最初出现显著降低。目前的结果表明，所分析的两种胶原膜都经历了与多核巨细胞相关的血管化。两种材料均未发生跨膜血管化。微血管存在于预先存在的血管和脂肪岛骨架内。需要进一步的长期研究和临床研究来确定观察到的异物巨细胞如何影响组织再生。
