Mörtstedt Harriet, Ali Neserin, Kåredal Monica, Jacobsson Helene, Rietz Emelie, Diab Kerstin Kronholm, Nielsen Jörn, Jönsson Bo A G, Lindh Christian H
Division of Occupational and Environmental Medicine, Department of Laboratory Medicine, Lund University , SE-221 85 Lund, Sweden.
J Proteome Res. 2015 Feb 6;14(2):860-73. doi: 10.1021/pr5009306. Epub 2015 Jan 16.
Hairdressers have an increased risk for developing airway symptoms, for example, asthma and rhinitis. Persulfates, which are oxidizing agents in bleaching powder, are considered important causal agents for these symptoms. However, the underlying mechanisms are unclear. The aim was therefore to measure proteomic changes in nasal lavage fluid from persulfate-challenged subjects to identify proteins potentially involved in the pathogenesis of bleaching powder-associated rhinitis or candidate effect biomarkers for persulfate. Also, oxidized peptides were measured to evaluate their usefulness as biomarkers for persulfate exposure or effect, for example, oxidative stress. Samples from hairdressers with and without bleaching powder-associated rhinitis were analyzed with liquid chromatography tandem mass spectrometry using selected reaction monitoring to target 246 proteins and five oxidized peptides. Pathway analysis was applied to obtain a functional overview of the proteins. Several proteins involved in biologically meaningful pathways, functions, or disorders, for example, inflammatory responses, oxidative stress, epithelium integrity, and dermatological disorders, changed after the persulfate challenge. A list with nine proteins that appeared to be affected by the persulfate challenge and should be followed up was defined. An albumin peptide containing oxidized tryptophan increased 2 h and 5 h after the challenge but not after 20 min, which indicates that such peptides may be useful as oxidative stress biomarkers.
美发师患气道症状（如哮喘和鼻炎）的风险增加。过硫酸盐是漂粉中的氧化剂，被认为是这些症状的重要致病因素。然而，其潜在机制尚不清楚。因此，本研究旨在检测过硫酸盐激发后受试者鼻腔灌洗液中的蛋白质组变化，以鉴定可能参与漂粉相关鼻炎发病机制的蛋白质或过硫酸盐的候选效应生物标志物。此外，还检测了氧化肽，以评估其作为过硫酸盐暴露或效应（如氧化应激）生物标志物的效用。使用选择反应监测的液相色谱串联质谱法对有和无漂粉相关鼻炎的美发师样本进行分析，以靶向246种蛋白质和5种氧化肽。应用通路分析以获得蛋白质的功能概述。过硫酸盐激发后，几种参与生物学意义通路、功能或疾病（如炎症反应、氧化应激、上皮完整性和皮肤病）的蛋白质发生了变化。定义了一份似乎受 过硫酸盐激发影响且应进一步研究的9种蛋白质清单。一种含有氧化色氨酸的白蛋白肽在激发后2小时和5小时增加，但在20分钟后未增加，这表明此类肽可能作为氧化应激生物标志物有用。
