Orsi Gianni, De Maria Carmelo, Montemurro Francesca, Chauhan Veeren M, Aylott Jonathan W, Vozzi Giovanni
Largo Lucio Lazzarino 1, 56122, Pisa, Italy.
Curr Top Med Chem. 2015;15(3):271-8. doi: 10.2174/1568026614666141229114738.
Today biomedical sciences are experiencing the importance of imaging biological parameters with luminescence methods. Studying 2D pH distribution with those methods allows building knowledge about complex cellular processes. Immobilizing pH sensitive nanoparticles inside hydrogel matrixes, in order to guarantee a proper SNR, could easily make stable and biocompatible 2D sensors. Inkjet printing is also well known as tool for printing images onto porous surfaces. Recently it has been used as a free-form fabrication method for building three-dimensional parts, and now is being explored as a way of printing electrical and optical devices. Inkjet printing was used either as a rapid prototyping method for custom biosensors. Sol-gel method is naturally bound with inkjet, because the picoliter-sized ink droplets evaporate quickly, thus allowing quick sol-gel transitions on the printed surface. In this work will be shown how to merge those technologies, in order to make a nanoparticles doped printable hydrogel, which could be used for making 2D/3D smart scaffolds able to monitor cell activities. An automated image analysis system was developed in order to quickly have the pH measurements from pH nanosensors fluorescence images.
如今，生物医学科学正体验着利用发光方法对生物参数进行成像的重要性。用这些方法研究二维pH分布有助于建立关于复杂细胞过程的知识。将pH敏感纳米颗粒固定在水凝胶基质中，以保证合适的信噪比，能够轻松制造出稳定且生物相容的二维传感器。喷墨打印作为一种将图像打印到多孔表面的工具也广为人知。最近，它已被用作制造三维部件的自由成型制造方法，并且目前正被探索作为一种打印电气和光学设备的方式。喷墨打印被用作定制生物传感器的快速原型制作方法。溶胶 - 凝胶法与喷墨自然结合，因为皮升大小的墨滴蒸发迅速，从而允许在打印表面快速进行溶胶 - 凝胶转变。在这项工作中，将展示如何融合这些技术，以制造出掺杂纳米颗粒的可打印水凝胶，其可用于制造能够监测细胞活动的二维/三维智能支架。为了从pH纳米传感器荧光图像快速获得pH测量值，开发了一个自动图像分析系统。
