Wu Yun, Ge Honghua, Zhou Zhongze
School of Resources and Environmental Engineering, Anhui University, No. 111, Jiulong Road, Hefei, 230601, China.
Environ Sci Pollut Res Int. 2015 Jun;22(11):8471-9. doi: 10.1007/s11356-014-3997-y. Epub 2014 Dec 30.
Agricultural waste has been used in the treatment of cyanobacterial bloom because of its environmental friendly and cost-efficient characteristics. In this work, the effects of Fructus ligustri lucidi (FLL) on the growth inhibition, physiological properties, algicidal property, and cell ultrastructure of Microcystis aeruginosa were investigated for the first time. The alga was efficiently inhibited by FLL at the dosages from 0.25 to 4.0 g L(-1), and the Chl-a fluorescence and metabolic activity of cells also declined gradually. During 25 days incubation time, the inhibition ratio of 0.25 g L(-1) dosage increased from 8 to 68 %, the percentage of intact cells decreased from 94.4 to 59.8 %, the inhibition ratio of 2.0 and 4.0 g L(-1) dosages was nearly 100 %, and the cell membranes were completely broken. The results of Chl-a, propidium iodide (PI) staining, fluorescein diacetate (FDA) staining, and transmission electron microscopy (TEM) assays were consistent with that of growth inhibition tests. The new medium test with the PI staining test suggested that FLL may act as an algicidal agent which can inhibit the growth of M. aeruginosa in the acute time. Consequently, FLL could be an excellent choice in the treatment of eutrophic water.
由于农业废弃物具有环境友好和成本效益高的特点，已被用于处理蓝藻水华。在本研究中，首次研究了女贞子（FLL）对铜绿微囊藻的生长抑制、生理特性、杀藻性能和细胞超微结构的影响。FLL在0.25至4.0 g L(-1)剂量下能有效抑制该藻类，细胞的叶绿素a荧光和代谢活性也逐渐下降。在25天的培养时间内，0.25 g L(-1)剂量的抑制率从8%增加到68%，完整细胞百分比从94.4%下降到59.8%，2.0和4.0 g L(-1)剂量的抑制率接近100%，细胞膜完全破裂。叶绿素a、碘化丙啶（PI）染色、荧光素二乙酸酯（FDA）染色和透射电子显微镜（TEM）分析结果与生长抑制试验结果一致。PI染色试验的新培养基试验表明，FLL可能作为一种杀藻剂，在短时间内抑制铜绿微囊藻的生长。因此，FLL可能是富营养化水体处理的一个极佳选择。
