Zhejiang Province Key Laboratory for Water Pollution Control and Environmental Safety, Hangzhou, PR China.
Chemosphere. 2010 Nov;81(9):1118-23. doi: 10.1016/j.chemosphere.2010.09.001. Epub 2010 Oct 8.
The current trend of global warming is expected to stimulate the expansion of harmful cyanobacteria blooms. Previously, the occidental type of barley straw has been used to control blooms in Europe and America, but very little is known about the antialgal abilities of its oriental relative. We tested the use of Tibetan hulless barley straw--the progenitor of oriental barley--to inhibit the growth of cyanobacterium Microcystis aeruginosa. Flow cytometry allowed assessment at single-cell level, with morphologic parameters (cell volume, cell membrane integrity) and physiological parameters (in vivo Chlorophyll a fluorescence, metabolic activity) used as endpoints. The reduction of cell densities together with integrated cell membranes suggests that Tibetan barley may act as an algistatic agent. Doses from 2.0 to 8.0 g L⁻¹ of Tibetan barley straw efficiently inhibited the alga, but these doses were much higher than those of occidental barley. Such a large dosage introduced additional nutrients, which stimulated the intracellular metabolic activity and induced two physiological subpopulations in the acute term. After mid and long-term exposure, the growth inhibition effect exceeded the stimulation effect, so that the cells' metabolic activity and Chlorophyll a fluorescence decayed, simultaneously with shrinkage in the algal cell volume.
当前全球变暖的趋势预计会刺激有害蓝藻水华的扩张。以前，西方类型的大麦秸秆曾被用于欧美控制水华，但对于其东方亲缘种的抑藻能力知之甚少。我们测试了使用西藏青稞秸秆——东方大麦的祖先——来抑制铜绿微囊藻的生长。流式细胞术可以在单细胞水平上进行评估，使用形态参数（细胞体积、细胞膜完整性）和生理参数（体内叶绿素 a 荧光、代谢活性）作为终点。细胞密度的降低以及整合的细胞膜表明，西藏青稞可能具有抑藻作用。2.0 到 8.0 g L⁻¹ 的青稞秸秆剂量有效地抑制了藻类，但这些剂量远高于西方大麦的剂量。如此大的剂量引入了额外的营养物质，这在急性条件下刺激了细胞内的代谢活性，并诱导了两个生理亚群。在中期和长期暴露后，生长抑制作用超过了刺激作用，导致细胞的代谢活性和叶绿素 a 荧光衰减，同时藻类细胞体积缩小。
