Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, MOE, School of Environment, Hohai University, Nanjing, China.
Chemosphere. 2013 Apr;91(5):608-15. doi: 10.1016/j.chemosphere.2012.12.064. Epub 2013 Jan 24.
The objectives of this work were to prepare and characterize an anti-algal sustained-release granule, then study its mode of action on Microcystis aeruginosa. The anti-algal sustained-release granule was prepared with artemisinin using alginate-chitosan microcapsule technology and characterized by a high performance liquid chromatography with an evaporative light-scattering detector, Fourier transform infrared spectral analysis, and a scanning electron microscope. The optimum preparation (in %, w/v) using the orthogonal method was: 2.5 sodium alginate; 0.25 chloride; 0.6 artemisinin; 2 calcium chloride; and 1.5 mL of the cross-linking agent, glutaraldehyde. These artemisinin sustained-release granules had a high encapsulation efficiency (up to 68%) and good release properties (release time of more than 40 d). Artemisinin sustained-release granules released cumulatively in a solution containing M. aeruginosa, and the stress on algae increased gradually within 30 d. Artemisinin sustained-release granules decreased the content of the soluble protein, Chlorophyll a in 30 d, increased the superoxide dismutase activity of M. aeruginosa, but exerted no effect on the soluble sugar content. Compared to direct dosing of artemisinin, algae can be inhibited longer and more effectively by the artemisinin sustained-release granules. The results of our research can aid in the development of new anti-algal sustained-release granules and lead to further study of their application in the field.
本工作旨在制备和表征一种抗藻缓释颗粒，并研究其对铜绿微囊藻的作用模式。采用海藻酸钠-壳聚糖微胶囊技术，以青蒿素为芯材，高效液相色谱-蒸发光散射检测器、傅里叶变换红外光谱分析和扫描电子显微镜对其进行了表征。采用正交试验法优化的最佳制备条件（w/v，%）为：2.5 海藻酸钠；0.25 氯化钙；0.6 青蒿素；2 氯化钙；交联剂戊二醛 1.5 mL。该青蒿素缓释颗粒包封率高（高达 68%），释放性能良好（释放时间超过 40 d）。青蒿素缓释颗粒在含有铜绿微囊藻的溶液中累积释放，30 d 内对藻类的胁迫逐渐增加。青蒿素缓释颗粒在 30 d 内降低了可溶性蛋白和叶绿素 a 的含量，增加了铜绿微囊藻的超氧化物歧化酶活性，但对可溶性糖含量没有影响。与直接投加青蒿素相比，青蒿素缓释颗粒能更有效地长时间抑制藻类。本研究结果可为新型抗藻缓释颗粒的开发提供参考，并进一步推动其在该领域的应用研究。
