Sadek Shimaa A, Soliman Amel M, Marzouk Mohamed
Department of Zoology, Faculty of Science, Cairo University, Cairo, Egypt.
Department of Zoology, Faculty of Science, Cairo University, Cairo, Egypt
Toxicol Ind Health. 2016 Aug;32(8):1358-1372. doi: 10.1177/0748233714561075. Epub 2014 Dec 29.
This study aims to evaluate the possible ameliorative effect of earthworm (Allolobophora caliginosa) extract (EE) against silicon dioxide nanoparticles (SiNPs)-induced liver injury in male albino rats. The effectiveness of EE was compared with silymarin as a standard hepatoprotective drug. The present work demonstrates the antioxidant activity of EE by 1,1-diphenyl-2-picrylhydrazyl assay. Administration of SiNPs, for 15 consecutive days, caused changes in most of the biochemical parameters, namely, serum aminotransferase enzymes activities (alanine transaminase and aspartate transaminase), alkaline phosphatase activity, total protein, total and direct bilirubin level, malondialdehyde, glutathione reduced, catalase, superoxide dismutase, glutathione reductase, and glutathione peroxidase. In addition, administration of SiNPs induced changes in liver tissue architecture. Administration of EE, for subsequent 30 days, to SiNPs exposure demonstrated significant ameliorative effects on nearly all the studied parameters, and such effects were compatible with those of silymarin. In addition, the administration of EE repairs, to some extent, the abnormal architecture of the liver tissue induced by SiNPs.
本研究旨在评估蚯蚓（背暗异唇蚓）提取物（EE）对二氧化硅纳米颗粒（SiNPs）诱导的雄性白化大鼠肝损伤的可能改善作用。将EE的有效性与作为标准保肝药物的水飞蓟宾进行比较。本研究通过1,1-二苯基-2-苦基肼法证明了EE的抗氧化活性。连续15天给予SiNPs会导致大多数生化参数发生变化，即血清氨基转移酶活性（丙氨酸转氨酶和天冬氨酸转氨酶）、碱性磷酸酶活性、总蛋白、总胆红素和直接胆红素水平、丙二醛、还原型谷胱甘肽、过氧化氢酶、超氧化物歧化酶、谷胱甘肽还原酶和谷胱甘肽过氧化物酶。此外，给予SiNPs会引起肝组织结构的变化。在暴露于SiNPs后连续30天给予EE对几乎所有研究参数均显示出显著的改善作用，且这些作用与水飞蓟宾的作用相当。此外，给予EE在一定程度上修复了由SiNPs诱导的肝组织异常结构。
