Ahmed Mohamed M, Hammad Amany A, Orabi Sahar H, Elbaz Hamed T, Elweza Ahmed E, Tahoun Enas A, Elseehy Mona M, El-Shehawi Ahmed M, Mousa Ahmed A
Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Menoufia, Egypt.
Department of Theriogenology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Menoufia, Egypt.
Animals (Basel). 2022 Jul 4;12(13):1723. doi: 10.3390/ani12131723.
This study examined the protective effect of earthworm extract (EE) on acrylamide (ACR)-induced reproductive dysfunction. Forty male rats were allocated into four groups = 10). The G I (control) group received distilled water (D.W.). The G II group received ACR (5 mg kg B.W. in D.W.) 5 days per week, orally, for 3 weeks. The G III group was administered EE (300 mg kg B.W in D.W.) 5 days per week, orally, for 3 weeks. The G IV group was pretreated with EE for 3 weeks and then co-treated with EE and ACR for an additional 3 weeks. ACR decreased the number of sperm, sperm viability, and total motility. However, it increased testosterone levels with no effect on the FSH or LH levels. Moreover, ACR increased the concentrations of malondialdehyde (MDA) and nitric oxide (NO). Meanwhile, it decreased the glutathione (GSH) concentration in testicular tissues. Notably, the expression levels of p53 and Ki-67 were increased in the degenerated spermatogenic cells and in the hyperplastic Leydig cells of the testis of the ACR-treated group, respectively. Acrylamide induced alterations in the testicular tissue architecture. Interestingly, EE restored the sperm parameters and recovered the testicular histological structures and the biochemical alterations induced by ACR. In conclusion, earthworm extract ameliorated ACR-induced reproductive toxicity via restoring the testicular antioxidant balance and suppressing p53 and Ki-67 expressions in testicular tissues.
本研究考察了蚯蚓提取物（EE）对丙烯酰胺（ACR）诱导的生殖功能障碍的保护作用。将40只雄性大鼠分为四组（每组 = 10只）。GI（对照组）接受蒸馏水（D.W.）。GII组每周5天口服ACR（5 mg/kg体重，溶于D.W.），持续3周。GIII组每周5天口服EE（300 mg/kg体重，溶于D.W.），持续3周。GIV组先用EE预处理3周，然后再用EE和ACR联合处理3周。ACR降低了精子数量、精子活力和总运动能力。然而，它提高了睾酮水平，对促卵泡生成素（FSH）或促黄体生成素（LH）水平没有影响。此外，ACR增加了丙二醛（MDA）和一氧化氮（NO）的浓度。同时，它降低了睾丸组织中谷胱甘肽（GSH）的浓度。值得注意的是，p53和Ki-67的表达水平在ACR处理组睾丸的退化生精细胞和增生的睾丸间质细胞中分别升高。丙烯酰胺引起睾丸组织结构改变。有趣的是，EE恢复了精子参数，恢复了睾丸组织结构以及ACR诱导的生化改变。总之，蚯蚓提取物通过恢复睾丸抗氧化平衡以及抑制睾丸组织中p53和Ki-67的表达，改善了ACR诱导的生殖毒性。
