Schmidt J, Aubele M, Jütting U, Rodenacker K, Luz A, Erfle V, Burger G
GSF-Abteilung für Molekulare Zellpathologie, Institut für Strahlenschutz, Neuherberg, Federal Republic of Germany.
Biochem Biophys Res Commun. 1989 Oct 31;164(2):728-35. doi: 10.1016/0006-291x(89)91520-9.
Established osteoblast-like (OB) cells infected with the bone tumor-inducing C-type retrovirus OA MuLV remained nontumorigenic over 104 cell culture passages. DNA histograms revealed a new cell population with a stem line peak at 5c. A second OA MuLV-infected OB cell line underwent neoplastic transformation with increasing passage level. These cells showed diffuse aneuploidy. Stepwise linear discriminant analysis of the chromatin structure of control, OA MuLV-infected, and FBR osteosarcoma virus-transformed cell lines resulted in various levels of discrimination ranging between 79.6% for control cells versus nontumorigenic OA MuLV-infected cells, and 96.6% for nontumorigenic OA MuLV-infected cells versus FBR osteosarcoma virus-transformed cells. OA MuLV-infected tumorigenic cells and FBR osteosarcoma virus-transformed cells were discriminated at a 93.6% level.
感染骨肿瘤诱导性C型逆转录病毒OA MuLV的成骨细胞样（OB）细胞在超过104代细胞培养传代过程中仍无致瘤性。DNA直方图显示出一个新的细胞群体，其干细胞系峰位于5c处。第二个感染OA MuLV的OB细胞系随着传代水平的增加发生了肿瘤转化。这些细胞表现出弥漫性非整倍体。对对照、感染OA MuLV和FBR骨肉瘤病毒转化的细胞系的染色质结构进行逐步线性判别分析，得到了不同水平的判别结果，对照细胞与非致瘤性感染OA MuLV的细胞之间的判别率为79.6%，非致瘤性感染OA MuLV的细胞与FBR骨肉瘤病毒转化的细胞之间的判别率为96.6%。感染OA MuLV的致瘤性细胞和FBR骨肉瘤病毒转化的细胞之间的判别率为93.6%。
