细胞核染色质的计算机辅助成像细胞术揭示了骨肿瘤病毒感染及贴壁成骨样细胞的肿瘤转化。

Computer-assisted imaging cytometry of nuclear chromatin reveals bone tumor virus infection and neoplastic transformation of adherent osteoblast-like cells.

作者信息

Schmidt J, Aubele M, Jütting U, Rodenacker K, Luz A, Erfle V, Burger G

机构信息

GSF-Abteilung für Molekulare Zellpathologie, Institut für Strahlenschutz, Neuherberg, Federal Republic of Germany.

出版信息

Biochem Biophys Res Commun. 1989 Oct 31;164(2):728-35. doi: 10.1016/0006-291x(89)91520-9.

DOI:10.1016/0006-291x(89)91520-9

PMID:2554899

Abstract

Established osteoblast-like (OB) cells infected with the bone tumor-inducing C-type retrovirus OA MuLV remained nontumorigenic over 104 cell culture passages. DNA histograms revealed a new cell population with a stem line peak at 5c. A second OA MuLV-infected OB cell line underwent neoplastic transformation with increasing passage level. These cells showed diffuse aneuploidy. Stepwise linear discriminant analysis of the chromatin structure of control, OA MuLV-infected, and FBR osteosarcoma virus-transformed cell lines resulted in various levels of discrimination ranging between 79.6% for control cells versus nontumorigenic OA MuLV-infected cells, and 96.6% for nontumorigenic OA MuLV-infected cells versus FBR osteosarcoma virus-transformed cells. OA MuLV-infected tumorigenic cells and FBR osteosarcoma virus-transformed cells were discriminated at a 93.6% level.

摘要

感染骨肿瘤诱导性C型逆转录病毒OA MuLV的成骨细胞样（OB）细胞在超过104代细胞培养传代过程中仍无致瘤性。DNA直方图显示出一个新的细胞群体，其干细胞系峰位于5c处。第二个感染OA MuLV的OB细胞系随着传代水平的增加发生了肿瘤转化。这些细胞表现出弥漫性非整倍体。对对照、感染OA MuLV和FBR骨肉瘤病毒转化的细胞系的染色质结构进行逐步线性判别分析，得到了不同水平的判别结果，对照细胞与非致瘤性感染OA MuLV的细胞之间的判别率为79.6%，非致瘤性感染OA MuLV的细胞与FBR骨肉瘤病毒转化的细胞之间的判别率为96.6%。感染OA MuLV的致瘤性细胞和FBR骨肉瘤病毒转化的细胞之间的判别率为93.6%。