二十碳五烯酸和二十二碳六烯酸对人肺癌中前列腺素E2合成的体外作用

In vitro effect of eicosapentaenoic and docosahexaenoic acids on prostaglandin E2 synthesis in a human lung carcinoma.

作者信息

de Antueno R J, de Bravo M G, Toledo J, Mercuri O, De Tomás M E

机构信息

Instituto de Investigaciones Bioquímicas de La Plata Facultad de Medicina, Argentina.

出版信息

Biochem Int. 1989 Sep;19(3):489-96.

PMID:2554913

Abstract

The capacity to synthesize both prostaglandins E1 (PGE1) and E2 (PGE2) has been determined in human lung mucoepidermoid carcinoma homogenates when [14C]-fatty acid precursors were added to the incubation medium. Only 10% of the total radioactivity recovered in PGs was found in PGF1 alpha and PGF2 alpha. The experiments were principally focused to inhibit the PGE2 synthesis either with pure eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids or with mixtures of both n-3 fatty acids obtained from fish oil. The results demonstrated that significant inhibitions were found when using 25 microM or a higher concentration of pure EPA or DHA in the incubation medium; however, 5 microM of mixtures of different EPA/DHA ratio caused the same inhibition. The results suggest that EPA and DHA, when added together, may enforce their inhibitory effect on PGE2 synthesis.

摘要

当向孵育培养基中添加[14C] -脂肪酸前体时，已测定人肺黏液表皮样癌匀浆中合成前列腺素E1（PGE1）和E2（PGE2）的能力。在PGs中回收的总放射性中，仅10%存在于PGF1α和PGF2α中。实验主要集中于用纯二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）或从鱼油中获得的两种n - 3脂肪酸的混合物来抑制PGE2的合成。结果表明，当在孵育培养基中使用25μM或更高浓度的纯EPA或DHA时，发现有显著抑制作用；然而，5μM不同EPA/DHA比例的混合物也产生相同的抑制作用。结果表明，EPA和DHA一起添加时，可能会增强它们对PGE2合成的抑制作用。