Chen Yun, Zhou Yongjin J, Siewers Verena, Nielsen Jens
Systems and Synthetic Biology, Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, SE-412 96, Gothenburg, Sweden.
Adv Biochem Eng Biotechnol. 2015;148:143-60. doi: 10.1007/10_2014_284.
Microbial production of isoprenoids provides an attractive alternative to biomass extraction and chemical synthesis. Although widespread research aims for isoprenoid biosynthesis, it is still in its infancy in terms of delivering commercial products. Large barriers remain in realizing a cost-competitive process, for example, developing an optimal microbial cell factory. Here, we summarize the many tools and methods that have been developed in the metabolic engineering of isoprenoid production, with the advent of systems biology and synthetic biology, and discuss how these technologies advance to accelerate the design-build-test engineering cycle to obtain optimum microbial systems. It is anticipated that innovative combinations of new and existing technologies will continue to emerge, which will enable further development of microbial cell factories for commercial isoprenoid production.
微生物生产类异戊二烯为生物质提取和化学合成提供了一种有吸引力的替代方案。尽管对类异戊二烯生物合成的研究广泛开展，但在提供商业产品方面仍处于起步阶段。在实现具有成本竞争力的工艺方面仍存在巨大障碍，例如，开发一个优化的微生物细胞工厂。在此，我们总结了随着系统生物学和合成生物学的出现，在类异戊二烯生产代谢工程中所开发的众多工具和方法，并讨论这些技术如何推进以加速设计-构建-测试工程循环，从而获得最佳的微生物系统。预计新技术与现有技术的创新组合将不断涌现，这将推动用于商业类异戊二烯生产的微生物细胞工厂的进一步发展。
