Wei J S, Huang H M, Shyu W C, Wu C S
Department of Biochemistry, Chang Gung Medical College, Taoyuan, Taiwan, R.O.C.
Clin Chem. 1989 Nov;35(11):2247-9.
In this simple method for rapid enzymatic determination of total cholesterol in human gallstones, gallstone powder is dissolved in an 80/20 by vol mixture of N,N-dimethylformamide and dimethyl sulfoxide and reacted directly with the aqueous enzymatic reagent, without further treatment. The organic solvents do not interfere with enzymatic activities of cholesterol oxidase, esterase, and peroxidase in the assay mixture. The method is reproducible (CVs 3.7% to 6.6%), analytical recoveries ranged from 98.6% to 102%, and linearity is good. Furthermore, results correlated well with those obtained by infrared spectroscopic measurements.
在这种用于快速酶法测定人胆结石中总胆固醇的简单方法中，胆结石粉末溶解于体积比为80/20的N，N - 二甲基甲酰胺和二甲基亚砜混合液中，并直接与水性酶试剂反应，无需进一步处理。有机溶剂不会干扰测定混合物中胆固醇氧化酶、酯酶和过氧化物酶的酶活性。该方法具有可重复性（变异系数为3.7%至6.6%），分析回收率在98.6%至102%之间，线性良好。此外，结果与通过红外光谱测量获得的结果相关性良好。
