一种用于测定脂质提取物中胆固醇的灵敏酶促测定法。
A sensitive enzymatic assay for determination of cholesterol in lipid extracts.
作者信息
Omodeo Salè F, Marchesini S, Fishman P H, Berra B
出版信息
Anal Biochem. 1984 Nov 1;142(2):347-50. doi: 10.1016/0003-2697(84)90475-5.
PMID:6528973
Abstract
A procedure for the determination of free and total cholesterol in lipid extracts is described. The method for free cholesterol employs cholesterol oxidase to generate H2O2 and peroxidase to catalyze the reaction of H2O2 with o-dianisidine to yield a colored product. For the determination of total cholesterol, cholesterol ester hydrolase is included.
摘要
本文描述了一种测定脂质提取物中游离胆固醇和总胆固醇的方法。游离胆固醇的测定方法是利用胆固醇氧化酶生成过氧化氢，并用过氧化物酶催化过氧化氢与邻联茴香胺的反应以产生有色产物。对于总胆固醇的测定，则加入了胆固醇酯水解酶。
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