Chen Jianping, Yang Jing, Jiang Jingting, Zhuang Yun, He Wei
Department of Gastroenterology, The Third Affiliated Hospital of Soochow University, The First People's Hospital of Changzhou Changzhou, Jiangsu, China.
Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University, The First People's Hospital of Changzhou Changzhou, Jiangsu, China.
Int J Clin Exp Pathol. 2014 Oct 15;7(11):8304-11. eCollection 2014.
Dendritic cells (DCs) and natural killer (NK) cells initiate specific immune responses against tumor cells. The aim of the present study was to determine the cytotoxicity and the subsets of the DC and NK cells and the cytokines level of DC and NK cells from cancer tissue and peripheral blood in the gastric cancer patients. Cytotoxicity of DC and NK was determined using the Cytotox non-radioactive assay. The cytotoxic activity of DC or NK isolated from cancer tissue and peripheral blood was attenuated in gastric cancer patients. CD11c, CD80, CD83, CD16, CD57 and CD69 were decreased in the cancer tissue and peripheral blood in the gastric cancer patients. CD86, CCR7 and CD59 were no significance in the cancer tissue and peripheral blood from gastric cancer patients. Tumor necrosis factor (TNF)-α, interleukin (IL)-2, T-bet and IL-15Rβ levels were decreased in DC and NK from the gastric cancer tissue and peripheral blood in the gastric cancer patients. IL-15 and IL-15Rα level were no significance in DC and NK in the gastric cancer tissue and peripheral blood in the gastric cancer patients. These results indicate that the cytotoxic activity and subsets and cytokines of DC and NK cells in the cancer tissue and peripheral blood in the gastric cancer patients were decreased. The decrease of subsets content and cytokines of DC and NK may contribute to a decrease in the function of DC and NK in the tissue and peripheral blood in the gastric cancer patients.
树突状细胞（DCs）和自然杀伤（NK）细胞启动针对肿瘤细胞的特异性免疫反应。本研究的目的是确定胃癌患者癌组织和外周血中DC和NK细胞的细胞毒性、亚群以及DC和NK细胞的细胞因子水平。使用细胞毒性非放射性检测法测定DC和NK的细胞毒性。从胃癌患者的癌组织和外周血中分离出的DC或NK的细胞毒性活性减弱。胃癌患者癌组织和外周血中的CD11c、CD80、CD83、CD16、CD57和CD69减少。CD86、CCR7和CD59在胃癌患者的癌组织和外周血中无显著差异。胃癌患者癌组织和外周血中DC和NK的肿瘤坏死因子（TNF）-α、白细胞介素（IL）-2、T-bet和IL-15Rβ水平降低。IL-15和IL-15Rα水平在胃癌患者癌组织和外周血中的DC和NK中无显著差异。这些结果表明，胃癌患者癌组织和外周血中DC和NK细胞的细胞毒性活性、亚群及细胞因子均降低。DC和NK亚群含量及细胞因子的减少可能导致胃癌患者组织和外周血中DC和NK功能下降。
