伊朗霍拉桑拉扎维省丙型肝炎病毒的基因型分布。

Genotype distribution of hepatitis C virus in Khorasan Razavi Province, Iran.

机构信息

Department of Clinical Toxicology, Mashhad University of Medical Sciences, Iran.

出版信息

Turk J Med Sci. 2014;44(4):656-60. doi: 10.3906/sag-1305-64.

PMID:25551938

Abstract

BACKGROUND/AIM: Several types of the hepatitis C virus (HCV), with variations in different parts of the genome, have been isolated from different regions of the world. Based on heterogenic sequences in the isolated genome, HCV is classified into different genotypes and subtypes. Data on distribution of HCV genotypes in a certain region could be important to patient management. Therefore, this study was conducted to determine the distribution of HCV in Mashhad, Northeast Iran.

MATERIALS AND METHODS

This cross-sectional study was conducted on 103 patients with HCV infections in Mashhad. Among the participants, at least 22 (21.4%) were intravenous drug users. HCV seropositivity was determined by an enzyme-linked immunosorbent assay and was confirmed by reverse transcriptase polymerase chain reaction. HCV-positive samples were selected for HCV genotyping using genotype specific primers.

RESULTS

Of 103 subjects, 43 (41.7%) and 34 (33.0%) had genotypes 1a and 3a, respectively. Other genotypes including 1b, 2a, 2b, 3b, and 5a were found in 4 (3.9%), 1 (1.0%), 3 (2.9%), 4 (3.9%), and 1 (1.0%), respectively. Coinfections with 2 genotypes were also observed in 11 (10.7%) patients. Genotyping for 2 (1.9%) of 103 samples did not produce any results.

CONCLUSION

Genotypes la and 3a were found to be the most prevalent HCV genotypes in Mashhad, Iran.

摘要

背景/目的：从世界不同地区分离出了几种丙型肝炎病毒（HCV），它们在基因组的不同部位存在变异。根据分离基因组中的异质序列，HCV 被分为不同的基因型和亚型。特定地区 HCV 基因型的分布数据对患者管理可能很重要。因此，本研究旨在确定伊朗东北部马什哈德 HCV 的分布情况。

材料与方法

这是一项在马什哈德的 103 名 HCV 感染者中进行的横断面研究。在参与者中，至少有 22 名（21.4%）为静脉吸毒者。采用酶联免疫吸附试验检测 HCV 血清阳性，并通过逆转录聚合酶链反应进行确认。选择 HCV 阳性样本，使用基因型特异性引物进行 HCV 基因分型。

结果

在 103 例患者中，43 例（41.7%）和 34 例（33.0%）分别为基因型 1a 和 3a。其他基因型包括 1b、2a、2b、3b 和 5a，分别在 4 例（3.9%）、1 例（1.0%）、3 例（2.9%）、4 例（3.9%）和 1 例（1.0%）中发现。11 例（10.7%）患者存在两种基因型的合并感染。对 103 例样本中的 2 例（1.9%）进行基因分型未得到任何结果。