Sepahi Samaneh, Pasdar Alireza, Ahadi Mitra, Gerayli Sina, Rostami Sina, Meshkat Zahra
1 Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad , Mashhad, Iran .
Viral Immunol. 2014 Oct;27(8):398-403. doi: 10.1089/vim.2014.0024. Epub 2014 Aug 13.
High prevalence of hepatitis c virus (HCV) infection in some areas necessitates more investigations of the causative factors. Genetic factors that cause disruption in operation or secretion of interleukin 10 (IL-10), an anti-inflammatory cytokine, may play a role in the intensity of the disease. The aim of this study was to evaluate genetic variants of IL-10 gene polymorphisms in HCV patients and their relationship with HCV disease. Fifty HCV patients and the same number of healthy individuals who were referred to hepatitis clinic in Mashhad, northeast of Iran, were recruited. Genomic DNA was extracted from whole blood. Genotyping for IL-10 gene promoter polymorphisms in three positions (-1082 G>A, -819 C>T and -592 C>A) was conducted by amplification refractory mutation system-polymerase chain reaction. Haplotype analysis was performed using PHASE software. In a recessive analysis model of the -1082 position (GG vs. AA+AG), GG genotype was more common in patients (adjusted p = 0.02; OR = 4.66 [95% CI 1.31-16.35]). Also, ATA haplotype was more prevalent in HCV patients (adjusted p = 0.061; OR = 1.87 [95% CI 0.97-3.61]). Also, ATC/GCA diplotypes were more common in controls (adjusted p=0.002; adjusted OR = 0.27 [95% CI 0.11-0.63]). Although we found a possible association between IL-10 promoter polymorphisms and HCV infection, certain genotypes or diplotypes may confer a higher risk or susceptibility for developing HCV infection.
某些地区丙型肝炎病毒（HCV）感染的高流行率使得有必要对其致病因素进行更多调查。导致抗炎细胞因子白细胞介素10（IL-10）的运作或分泌受到干扰的遗传因素可能在该疾病的严重程度中起作用。本研究的目的是评估HCV患者中IL-10基因多态性的遗传变异及其与HCV疾病的关系。招募了50名HCV患者和相同数量转诊至伊朗东北部马什哈德肝炎诊所的健康个体。从全血中提取基因组DNA。通过扩增阻滞突变系统-聚合酶链反应对IL-10基因启动子三个位置（-1082 G>A、-819 C>T和-592 C>A）的多态性进行基因分型。使用PHASE软件进行单倍型分析。在-1082位置的隐性分析模型（GG与AA+AG）中，GG基因型在患者中更常见（校正p = 0.02；OR = 4.66 [95% CI 1.31 - 16.35]）。此外，ATA单倍型在HCV患者中更普遍（校正p = 0.061；OR = 1.87 [95% CI 0.97 - 3.61]）。而且，ATC/GCA双倍型在对照组中更常见（校正p = 0.002；校正OR = 0.27 [95% CI 0.11 - 0.63]）。虽然我们发现IL-10启动子多态性与HCV感染之间可能存在关联，但某些基因型或双倍型可能会增加发生HCV感染的风险或易感性。
