Yan Ying, Chen Xumiao, Chen Xiangrui, Gao Feng, Al-Farraj Saleh A, Al-Rasheid Khaled A S
Laboratory of Protozoology, Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
Laboratory of Protozoology, Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Eur J Protistol. 2015 Feb;51(1):66-78. doi: 10.1016/j.ejop.2014.11.001. Epub 2014 Nov 11.
The present study investigates the morphologic and molecular characteristics of three Condylostoma species isolated from brackish and marine tropical habitats of China, including Condylostoma tropicum spec. nov., Condylostoma elongatum spec. nov. and Condylostoma curvum Burkovsky, 1970. The two new species have slender and elongated bodies with the posterior portion distinctly narrowed, forming long tails, thus they obviously differ from most congeners. In addition, Condylostoma tropicum spec. nov. is characterized by its small buccal cavity, single frontal cirrus, 26-33 somatic kineties, and moniliform macronucleus composed of 8-22 nodules. Condylostoma elongatum spec. nov. is distinguished by its huge body size (1000-1200μm long in vivo), the prominent buccal cavity, 5-7 frontal cirri and 37-43 somatic kineties. Condylostoma curvum is also reinvestigated in this paper using both protargol impregnation and molecular techniques. Phylogenetic analyses based on SSU rDNA sequence data indicate that the three organisms are located within the genus Condylostoma; the genus Condylostoma fails to form a monophyletic branch in both Maximum-likelihood tree and Bayesian inference analysis. Nonetheless, the AU test shows that the monophyly of Condylostoma could not be rejected.
本研究调查了从中国咸淡水和海洋热带生境中分离出的三种柱形虫的形态和分子特征，包括热带柱形虫新种、细长柱形虫新种和弯曲柱形虫（布尔科夫斯基，1970年）。这两个新种的身体细长，后部明显变窄，形成长尾，因此它们与大多数同属物种明显不同。此外，热带柱形虫新种的特征是口腔小、单一额纤毛、26 - 33列体纤毛以及由8 - 22个节结组成的念珠状大核。细长柱形虫新种的特点是体型巨大（活体长度为1000 - 1200μm）、口腔突出、5 - 7根额纤毛以及37 - 43列体纤毛。本文还使用原银浸染法和分子技术对弯曲柱形虫进行了重新研究。基于小亚基核糖体DNA序列数据的系统发育分析表明，这三种生物都属于柱形虫属；在最大似然树和贝叶斯推断分析中，柱形虫属均未形成单系分支。尽管如此，似然比检验表明柱形虫属的单系性不能被拒绝。
