Fernandes Noemi M, Dias Roberto J P, Schrago Carlos G, Silva-Neto Inácio D
Laboratório de Biologia Evolutiva Teórica e Aplicada, Dept. de Genética, Inst. de Biologia, CCS, Universidade Federal do Rio de Janeiro, CEP: 21941-617, Ilha do Fundão, Rio de Janeiro - RJ, Brazil.
Laboratório de Protozoologia, Dept. de Zoologia, ICB, Universidade Federal de Juiz de Fora, CEP: 36036-900, Juiz de Fora - MG, Brazil.
J Eukaryot Microbiol. 2015 Nov-Dec;62(6):722-32. doi: 10.1111/jeu.12228. Epub 2015 Jun 12.
Details on Condylostoma arenarium infraciliature have not been described; therefore, it is considered a poorly known species. The lack of detailed description on C. arenarium morphology caused several misidentifications that have accumulated in the literature. In this study, we present the first complete description of C. arenarium infraciliature based on protargol-impregnated organisms and scanning electron microscopy. We also have inferred the phylogenetic position of this species based on 18S rRNA sequences. The main characteristics of C. arenarium population from Guanabara Bay are as follows: in vivo elongated body shape with 350-600 μm length × 70-220 μm width, they are highly contractile when subjected to disturbances, green-yellowish cortical granules are present, contractile vacuoles absent, V-shaped peristome comprises approximately 1/5 of the total length, adoral zone with 83-145 membranelles, 1-2 small frontal cirrus observed only in impregnated specimens, 10-15 fiber-like stripes arranged transversely on the inner wall of the oral cavity, 30-45 somatic kineties, moniliform macronucleus with 15-20 nodules. Some observations on morphogenesis of C. arenarium were also included. In phylogenetic analyses, C. arenarium clustered with Condylostoma sp. within a clade composed of three C. curva sequences with high support values.
关于沙栖疣口虫纤毛下器的细节尚未被描述；因此，它被认为是一个鲜为人知的物种。由于缺乏对沙栖疣口虫形态的详细描述，文献中积累了一些错误鉴定。在本研究中，我们基于经原银浸染的生物体和扫描电子显微镜，首次完整描述了沙栖疣口虫的纤毛下器。我们还基于18S rRNA序列推断了该物种的系统发育位置。来自瓜纳巴拉湾的沙栖疣口虫种群的主要特征如下：活体时身体呈细长形，长350 - 600μm，宽70 - 220μm，受到干扰时高度可收缩，有黄绿色皮质颗粒，无收缩泡，V形口围约占全长的1/5，口前区有83 - 145片小膜，仅在浸染标本中观察到1 - 2根小额纤毛，口腔内壁横向排列着10 - 15条纤维状条纹，有30 - 45列体纤毛，念珠状大核有15 - 20个结节。还包括了一些关于沙栖疣口虫形态发生的观察。在系统发育分析中，沙栖疣口虫与疣口虫属的一个物种聚集在一个由三个具有高支持值的弯疣口虫序列组成的分支内。
