新型耐热克鲁维酵母在高固体负荷下对木质纤维素生物质进行脱木质素同步糖化发酵生产乙醇。
Simultaneous saccharification and fermentation of delignified lignocellulosic biomass at high solid loadings by a newly isolated thermotolerant Kluyveromyces sp. for ethanol production.
机构信息
Sardar Patel Renewable Energy Research Institute, P. Box No. 2, Vallabh Vidyanagar, 388 120 Gujarat, India.
Sardar Patel Renewable Energy Research Institute, P. Box No. 2, Vallabh Vidyanagar, 388 120 Gujarat, India.
出版信息
Bioresour Technol. 2015 Mar;179:331-338. doi: 10.1016/j.biortech.2014.11.116. Epub 2014 Dec 5.
Simultaneous saccharification and fermentation studies were carried out using thermotolerant newly isolated Kluyveromyces sp. with three different delignified lignocellulosic biomass viz. rice straw, wheat straw and sugarcane bagasse at 5-15% solid loading and 6-12 FPU g(-1) substrate enzyme loading for different time intervals 0-72 h at 42°C. Maximum ethanol achieved from rice straw, wheat straw and sugarcane bagasse with in-house crude cellulases from Aspergillus terreus was 23.23, 18.29 and 17.91 mg mL(-1) at 60 h with 10% solid load and 9 FPU g(-1) substrate enzyme loading. Tween 80 1% (v/v) enhanced the ethanol yield by 8.39%, 9.26% and 8.14% in rice straw, wheat straw and sugarcane bagasse, respectively. External supplementation of β-glucosidase to the crude as well commercial cellulases produced maximum theoretical ethanol yield of 71.76%, 63.77%, 57.15% and 84.56%, 72.47%, 70.55% from rice straw, wheat straw and sugarcane bagasse, respectively.
采用新型耐热克鲁维酵母，在 5-15%固含率和 6-12 FPU g(-1)底物酶负载下，对三种不同脱木质素木质纤维素生物质（水稻秸秆、小麦秸秆和甘蔗渣）进行同步糖化发酵研究，时间间隔为 0-72 h，温度为 42°C。用来自土曲霉的内源性粗纤维素酶，从水稻秸秆、小麦秸秆和甘蔗渣中获得的最大乙醇产量分别为 23.23、18.29 和 17.91 mg mL(-1)，在 10%固含率和 9 FPU g(-1)底物酶负载下发酵 60 h。吐温 80（1%，v/v）分别使水稻秸秆、小麦秸秆和甘蔗渣中的乙醇产量提高了 8.39%、9.26%和 8.14%。向粗酶和商业纤维素酶中添加 β-葡萄糖苷酶，可使水稻秸秆、小麦秸秆和甘蔗渣的最大理论乙醇产量分别达到 71.76%、63.77%、57.15%和 84.56%、72.47%、70.55%。
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