Shao Shiying, Guo Tiannan, Aebersold Ruedi
Division of Endocrinology, Tongji Hospital, Huazhong University of Science & Technology, Wuhan 430030, PR China; Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Wolfgang-Pauli-Str. 16, 8093, Switzerland.
Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Wolfgang-Pauli-Str. 16, 8093, Switzerland.
Biochim Biophys Acta. 2015 Jun;1854(6):519-27. doi: 10.1016/j.bbapap.2014.12.012. Epub 2014 Dec 30.
Diabetes mellitus (DM) is a metabolic disorder characterized by chronic hyperglycemia, which affects hundreds of millions of individuals worldwide. Early diagnosis and complication prevention of DM are helpful for disease treatment. However, currently available DM diagnostic markers fail to achieve the goals. Identification of new diabetic biomarkers assisted by mass spectrometry (MS)-based proteomics may offer solution for the clinical challenges. Here, we review the current status of biomarker discovery in DM, and describe the pressure cycling technology (PCT)-Sequential Window Acquisition of all Theoretical fragment-ion (SWATH) workflow for sample-processing, biomarker discovery and validation, which may accelerate the current quest for DM biomarkers. This article is part of a Special Issue entitled: Medical Proteomics.
糖尿病（DM）是一种以慢性高血糖为特征的代谢紊乱疾病，全球有数亿人受其影响。糖尿病的早期诊断和并发症预防有助于疾病治疗。然而，目前可用的糖尿病诊断标志物未能实现这些目标。基于质谱（MS）的蛋白质组学辅助鉴定新的糖尿病生物标志物可能为临床挑战提供解决方案。在此，我们综述了糖尿病生物标志物发现的现状，并描述了压力循环技术（PCT）-全理论碎片离子序列窗口采集（SWATH）工作流程，用于样品处理、生物标志物发现和验证，这可能会加速目前对糖尿病生物标志物的探索。本文是名为“医学蛋白质组学”的特刊的一部分。
