Wesselingh S L, Li Y W, Blessing W W
Department of Clinical Microbiology, University of S.A., Bedford Park.
Neurosci Lett. 1989 Nov 20;106(1-2):99-104. doi: 10.1016/0304-3940(89)90209-7.
Herpes simplex virus type 1 (HSV1) was injected into the rat adrenal gland. After 3 days the rat CNS was processed immunohistochemically to demonstrate viral antigen. In the lower thoracic spinal cord viral antigen was found in neurons in the intermediolateral column. In the medulla oblongata HSV1-positive neurons were found in the raphe pallidus and in the C1 and C3 regions of the rostral medulla. Approximately 50% of HSV1-positive neurons in the C1 and all the HSV1-positive neurons in the C3 area also contained phenylethanolamine N-methyltransferase (PNMT) and were thus identified as C1 and C3 cells. The HSV1-positive neurons in the C1 region which did not contain PNMT were also negative for tyrosine hydroxylase and were therefore not catecholamine-synthesizing neurons. The HSV1-positive neurons in the medulla oblongata were presumably transsynaptically labelled from the adrenal gland and our study therefore provides neuroanatomical evidence supporting the view that some C1 neurons are involved in controlling the function of the adrenal gland.
将1型单纯疱疹病毒（HSV1）注入大鼠肾上腺。3天后，对大鼠中枢神经系统进行免疫组织化学处理以显示病毒抗原。在下胸段脊髓，在中间外侧柱的神经元中发现了病毒抗原。在延髓，在苍白缝际以及延髓头端的C1和C3区域发现了HSV1阳性神经元。C1区域中约50%的HSV1阳性神经元和C3区域所有的HSV1阳性神经元也含有苯乙醇胺N-甲基转移酶（PNMT），因此被鉴定为C1和C3细胞。C1区域中不含PNMT的HSV1阳性神经元酪氨酸羟化酶也呈阴性，因此不是儿茶酚胺合成神经元。延髓中的HSV1阳性神经元可能是从肾上腺经突触标记的，因此我们的研究提供了神经解剖学证据，支持一些C1神经元参与控制肾上腺功能这一观点。
