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弯曲DNA的体外优先拓扑异构化

In vitro preferential topoisomerization of bent DNA.

作者信息

Caserta M, Amadei A, Di Mauro E, Camilloni G

机构信息

Centro di Studio per Gli Acidi Nucleici, CNR, Rome, Italy.

出版信息

Nucleic Acids Res. 1989 Nov 11;17(21):8463-74. doi: 10.1093/nar/17.21.8463.

DOI:10.1093/nar/17.21.8463
PMID:2555772
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC335019/
Abstract

The steps of the topoisomerization reaction by calf thymus DNA topoisomerase I on a DNA domain containing an intrinsically bent DNA sequence have been analyzed. High preferentiality of binding, cleavage and topoisomerization on the bent segment relative to the rest of the DNA domain was observed. These studies show the importance of the local DNA conformation in the reaction of eukaryotic DNA topoisomerase I and the interest of the use of this enzyme as a tool for the analysis of DNA conformation and DNA dynamics.

摘要

已对小牛胸腺DNA拓扑异构酶I在含有内在弯曲DNA序列的DNA结构域上进行拓扑异构化反应的步骤进行了分析。相对于DNA结构域的其余部分，在弯曲片段上观察到结合、切割和拓扑异构化的高度优先性。这些研究表明了局部DNA构象在真核生物DNA拓扑异构酶I反应中的重要性，以及使用这种酶作为分析DNA构象和DNA动力学工具的意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaa0/335019/fce9710dd282/nar00138-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaa0/335019/6ef6e1590dd2/nar00138-0069-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaa0/335019/3052188d8d01/nar00138-0070-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaa0/335019/9fc991a10494/nar00138-0072-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaa0/335019/5fc79abf8911/nar00138-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaa0/335019/fce9710dd282/nar00138-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaa0/335019/6ef6e1590dd2/nar00138-0069-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaa0/335019/3052188d8d01/nar00138-0070-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaa0/335019/9fc991a10494/nar00138-0072-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaa0/335019/5fc79abf8911/nar00138-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaa0/335019/fce9710dd282/nar00138-0075-a.jpg

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