牛（背最长肌）转录组中大理石花纹差异水平的测序和特征分析。
Sequencing and Characterization of Divergent Marbling Levels in the Beef Cattle (Longissimus dorsi Muscle) Transcriptome.
机构信息
College of Life Science, Shanxi Agriculture University, Taigu, Shanxi 030800, China.
Department of Animal Sciences, Washington State University, Pullman, WA 641227, USA.
出版信息
Asian-Australas J Anim Sci. 2015 Feb;28(2):158-65. doi: 10.5713/ajas.14.0394.
Marbling is an important trait regarding the quality of beef. Analysis of beef cattle transcriptome and its expression profile data are essential to extend the genetic information resources and would support further studies on beef cattle. RNA sequencing was performed in beef cattle using the Illumina High-Seq2000 platform. Approximately 251.58 million clean reads were generated from a high marbling (H) group and low marbling (L) group. Approximately 80.12% of the 19,994 bovine genes (protein coding) were detected in all samples, and 749 genes exhibited differential expression between the H and L groups based on fold change (>1.5-fold, p<0.05). Multiple gene ontology terms and biological pathways were found significantly enriched among the differentially expressed genes. The transcriptome data will facilitate future functional studies on marbling formation in beef cattle and may be applied to improve breeding programs for cattle and closely related mammals.
大理石花纹是牛肉质量的一个重要特征。分析肉牛转录组及其表达谱数据对于扩展遗传信息资源非常重要，并将支持对肉牛的进一步研究。使用 Illumina High-Seq2000 平台对肉牛进行 RNA 测序。从高大理石花纹（H）组和低大理石花纹（L）组中生成了约 2.5158 亿个清洁读取。所有样品中检测到约 19994 个牛基因（蛋白质编码）的 80.12%，根据折叠变化（>1.5 倍，p<0.05），749 个基因在 H 和 L 组之间表现出差异表达。在差异表达基因中发现了多个基因本体论术语和生物途径显著富集。转录组数据将有助于未来对牛肉大理石花纹形成的功能研究，并可应用于改进牛和相关哺乳动物的育种计划。
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