Muniz Maria Malane Magalhães, Simielli Fonseca Larissa Fernanda, Scalez Daiane Cristina Becker, Vega Aroa Suarez, Silva Danielly Beraldo Dos Santos, Ferro Jesus Aparecido, Chardulo Artur Loyola, Baldi Fernando, Cánovas Angela, de Albuquerque Lucia Galvão
School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp) Jaboticabal SP Brazil.
Department of Animal Biosciences Centre for Genetic Improvement of Livestock University of Guelph Guelph Ontario Canada.
Evol Appl. 2022 Mar 25;15(4):706-718. doi: 10.1111/eva.13365. eCollection 2022 Apr.
The aim of this study was to identify novel lncRNA differentially expressed (DE) between divergent animals for beef tenderness and marbling traits in Nellore cattle. muscle samples from the 20 most extreme bulls (of 80 bulls set) for tenderness, tender ( = 10) and tough ( = 10) groups, and marbling trait, high ( = 10) and low ( = 10) groups were used to perform transcriptomic analysis using RNA-Sequencing. For tenderness, 29 lncRNA were DE (-value ≤ 0.01) in tough beef animals in relation to tender beef animals. We observed that genic lncRNAs, for example, ., were overlapping exonic part of the gene, while . and . overlapped intronic part of the genes and . The lncRNA associated with , , and genes were enriched in the pathways associated with the ionotropic glutamate receptor, gamma-aminobutyric acid synthesis, and the ubiquitin-proteasome pathway. For marbling, 50 lncRNA were DE (-value ≤ 0.01) in high marbling group compared with low marbling animals. The genic lncRNAs, such as ., were overlapped exonic part of the gene, and the ., ., and . overlapped intronic parts of the and genes. The and genes were enriched in pathways associated with integrin signaling, which is involved in intracellular signals in response to the extracellular matrix, including cell form, mobility, and mediates progression through the cell cycle. In addition, the lincRNAs identified to marbling trait were associated with several genes related to calcium binding, muscle hypertrophy, skeletal muscle, lipase, and oxidative stress response pathways that seem to play a role important in the physiological processes related to meat quality. These findings bring new insights to better understand the biology mechanisms involved in the gene regulation of these traits, which will be valuable for a further investigation of the interactions between lncRNA and mRNAs, and of how these interactions may affect meat quality traits.
本研究的目的是鉴定内洛尔牛中与牛肉嫩度和大理石花纹性状差异相关的新型长链非编码RNA（lncRNA）。从80头公牛中挑选出20头在嫩度方面最极端的公牛（分为嫩肉组（n = 10）和老肉组（n = 10））以及大理石花纹性状方面最极端的公牛（分为高大理石花纹组（n = 10）和低大理石花纹组（n = 10）），采集其肌肉样本，利用RNA测序进行转录组分析。在嫩度方面，与嫩牛肉动物相比，老牛肉动物中有29个lncRNA差异表达（P值≤0.01）。我们观察到，基因lncRNA，例如……，与……基因的外显子部分重叠，而……和……与……基因和……基因的内含子部分重叠。与……、……和……基因相关的lncRNA在与离子型谷氨酸受体、γ-氨基丁酸合成以及泛素-蛋白酶体途径相关的通路中富集。在大理石花纹方面，与低大理石花纹动物相比，高大理石花纹组中有50个lncRNA差异表达（P值≤0.01）。基因lncRNA，如……，与……基因的外显子部分重叠，而……、……和……与……和……基因的内含子部分重叠。……和……基因在与整合素信号传导相关的通路中富集，整合素信号传导参与细胞对细胞外基质的反应中的细胞内信号传导，包括细胞形态、迁移，并介导细胞周期进程。此外，鉴定出的与大理石花纹性状相关的长链间核糖核酸（lincRNA）与几个与钙结合、肌肉肥大、骨骼肌、脂肪酶和氧化应激反应途径相关的基因有关，这些基因似乎在与肉质相关的生理过程中发挥重要作用。这些发现为更好地理解这些性状的基因调控所涉及的生物学机制带来了新的见解，这对于进一步研究lncRNA与mRNA之间的相互作用以及这些相互作用如何影响肉质性状将具有重要价值。
