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南极鱼类伯氏南极鱼(硬骨鱼纲,南极鱼亚目)头肾转录组的分析与表征:免疫相关基因的来源

Analysis and characterization of the head kidney transcriptome from the Antarctic fish Trematomus bernacchii (Teleostea, Notothenioidea): a source for immune relevant genes.

作者信息

Gerdol Marco, Buonocore Francesco, Scapigliati Giuseppe, Pallavicini Alberto

机构信息

Department of Life Sciences, University of Trieste, Via Giorgieri 5, 34127 Trieste, TS, Italy.

Department for Innovation in Biological, Agro-food and Forest Systems, University of Tuscia, Largo dell'Università snc, Viterbo, VT, Italy.

出版信息

Mar Genomics. 2015 Apr;20:13-5. doi: 10.1016/j.margen.2014.12.005. Epub 2014 Dec 31.

Abstract

In this study we describe the de novo assembled head kidney transcriptome of the Antarctic notothenioid fish Trematomus bernacchii, an important model species for biochemical, environmental and immunological studies. RNA-seq data was generated using Illumina paired-end sequencing, obtaining ~7 Gbp of sequence data, which were assembled into 96,641 contigs and annotated with the Trinotate pipeline. Since this sequence collection is expected to contain a relevant number of immunity-related transcripts, it will be used as a reference for future immunological studies in this species.

摘要

在本研究中,我们描述了南极南极鱼科鱼类伯氏南极鱼从头组装的头肾转录组,它是生化、环境和免疫学研究的重要模式物种。使用Illumina双端测序生成RNA-seq数据,获得了约7 Gbp的序列数据,这些数据被组装成96,641个重叠群,并使用Trinotate管道进行注释。由于预计该序列集合将包含大量与免疫相关的转录本,因此它将作为该物种未来免疫学研究的参考。

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