National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China.
Shanghai Engineering Research Center of Aquaculture, Shanghai 201306, China.
Zool Res. 2019 Jul 18;40(4):337-342. doi: 10.24272/j.issn.2095-8137.2019.028. Epub 2019 Apr 25.
Hemorrhagic septicemia is an acute, highly fatal disease that affects goldfish (). To gain a better understanding of related immune genes, the transcriptomes of the skin and head kidney of goldfish suffering hemorrhagic septicemia were sequenced, assembled, and characterized. Based on functional annotation, an extensive and diverse catalog of expressed genes were identified in both the skin and head kidney. As two different organs, pair-wise comparison identified 122/77 unigenes up/down-regulated (two-fold change with <0.05) in the skin and head kidney. Most genes of the immune pathways were expressed and isolated in both skin and head kidney, including interferon (IFN) transcription factors 1-10 and Toll-like receptors (TLRs). Interferon regulatory factor 3 (IRF3), a key IFN transcription factor, was up-regulated at the transcriptional level by polyriboinosinic: polyribocytidylic acid (poly I:C) challenge and regulated the IFN response by increasing the activity of IFN-β and IFN-stimulated response element (ISRE)-containing promoter. This study will benefit the identification and understanding of novel genes that play important roles in the immunological reactions of fish suffering from hemorrhagic septicemia.
出血性败血症是一种影响金鱼（）的急性、高致命性疾病。为了更好地了解相关免疫基因，对患有出血性败血症的金鱼的皮肤和头部肾脏进行了转录组测序、组装和特征分析。基于功能注释，在皮肤和头部肾脏中鉴定出了广泛而多样的表达基因目录。作为两个不同的器官，皮肤和头部肾脏之间的两两比较确定了 122/77 个基因上调/下调（变化倍数为 2 倍，<0.05）。大多数免疫途径的基因在皮肤和头部肾脏中均有表达和分离，包括干扰素（IFN）转录因子 1-10 和 Toll 样受体（TLRs）。干扰素调节因子 3（IRF3）是一种关键的 IFN 转录因子，多聚肌苷酸：多聚胞苷酸（poly I:C）挑战可在转录水平上调，通过增加 IFN-β 和 IFN 刺激反应元件（ISRE）含启动子的活性来调节 IFN 反应。本研究将有助于鉴定和了解在患有出血性败血症的鱼类的免疫反应中发挥重要作用的新基因。
