Shibuya H, Taniguchi T
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Nucleic Acids Res. 1989 Nov 25;17(22):9173-84. doi: 10.1093/nar/17.22.9173.
Regulated expression of interleukin-2 (IL-2) gene constitutes an essential part in the clonal proliferation of activated T lymphocytes (T-cells). In order to gain insight on the mechanism(s) of the IL-2 gene induction, deletions were introduced in the human IL-2 gene 5'-flanking region and the mitogen inducibility of each deletion mutant was examined in cultured T cell lines. At least four functional regions were identified, they contain potential binding sites for several transcription factors including one NF-kappa B and two octamer binding factor sites. Whereas each of the functional regions (or elements) is required for the maximal induction of the IL-2 gene by mitogen, one such region was found to be dispensable for activation by the HTLV-1-encoded trans-activator, tax-1. Furthermore, the potent immunosuppressive agent, cyclosporin A was found to inhibit the gene induction by mitogen, but not by tax-1.
白细胞介素-2（IL-2）基因的调控表达是活化T淋巴细胞（T细胞）克隆增殖的重要组成部分。为了深入了解IL-2基因诱导的机制，在人IL-2基因5'侧翼区域引入缺失，并在培养的T细胞系中检测每个缺失突变体的丝裂原诱导性。至少鉴定出四个功能区域，它们包含几个转录因子的潜在结合位点，包括一个核因子κB（NF-κB）和两个八聚体结合因子位点。虽然每个功能区域（或元件）对于丝裂原对IL-2基因的最大诱导是必需的，但发现其中一个这样的区域对于人嗜T淋巴细胞病毒1型（HTLV-1）编码的反式激活因子tax-1的激活是可有可无的。此外，发现强效免疫抑制剂环孢菌素A可抑制丝裂原诱导的基因，但不抑制tax-1诱导的基因。
