Ding Y A, Chang W K, Chen M L
Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Proc Natl Sci Counc Repub China B. 1989 Apr;13(2):102-8.
In the present study, we changed the fatty acid profile in blood and platelet membranes by dietary manipulation, and examined the effect on platelet aggregation in rats. Fifty-five rats were divided into five groups and fed for 56 days with 1% cholesterol and different types of fatty acid-rich diets: basal, lard, lard + fish oil, soybean oil, and soybean oil + fish oil. a decrease in serum arachidonic acid (20:4, omega-6, AA) and an increase in serum eicosapentaenoic acid (20:5, omega-3, EPA) were found in all experimental dietary groups fed with refined fish oil. Similar changes in the polyunsaturated fatty acids were also found in the platelet membrane phospholipids. Platelet aggregation, quantitated by the slope and height of the aggregation curve induced by different concentrations of ADP in a platelet aggregometer, was inhibited in all groups fed with refined fish oil. This inhibition of platelet aggregation may be related to an increase in the ratio of EPA and AA in the platelet membrane phospholipids after dietary manipulation. The differences in the platelet aggregation and thromboxane B2 (TXB2) concentration between the lard and the lard + fish oil groups were more profound than that between the soybean oil and the soybean oil + fish oil group. However, the malondialdehyde (MDA) concentration revealed no significant differences between the five groups.
在本研究中，我们通过饮食调控改变了血液和血小板膜中的脂肪酸谱，并检测了其对大鼠血小板聚集的影响。55只大鼠被分为五组，用含1%胆固醇和不同类型富含脂肪酸的饮食喂养56天：基础饮食、猪油、猪油+鱼油、大豆油以及大豆油+鱼油。在所有喂食精炼鱼油的实验饮食组中，均发现血清花生四烯酸（20:4，ω-6，AA）减少，血清二十碳五烯酸（20:5，ω-3，EPA）增加。在血小板膜磷脂中也发现了多不饱和脂肪酸的类似变化。通过血小板聚集仪中不同浓度ADP诱导的聚集曲线的斜率和高度来定量血小板聚集，结果显示所有喂食精炼鱼油的组中血小板聚集均受到抑制。这种血小板聚集的抑制可能与饮食调控后血小板膜磷脂中EPA与AA比例的增加有关。猪油组和猪油+鱼油组之间血小板聚集和血栓素B2（TXB2）浓度的差异比大豆油组和大豆油+鱼油组之间更为显著。然而，五组之间丙二醛（MDA）浓度没有显著差异。
