College of Fisheries, Henan Normal University, Xinxiang, China.
State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China.
FEBS Open Bio. 2019 Sep;9(9):1503-1520. doi: 10.1002/2211-5463.12695. Epub 2019 Jul 31.
Increases in atmospheric CO partial pressure have lowered seawater pH in marine ecosystems, a process called ocean acidification (OA). The effects of OA during the critical stages of larval development may have disastrous consequences for some marine species, including Babylonia areolata (Link 1807), a commercially important sea snail in China and South East Asia. To investigate how OA affects the proteome of Babylonia areolata, here we used label-free proteomics to study protein changes in response to acidified (pH 7.6) or ambient seawater (pH 8.1) during three larvae developmental stages of B. areolata, namely, the veliger larvae before attachment (E1), veliger larvae after attachment (E2), and carnivorous juvenile snail (E3). In total, we identified 720 proteins. This result suggested that acidification seriously affects late veliger stage after attachment (E2). Further examination of the roles of differentially expressed proteins, which include glutaredoxin, heat-shock protein 70, thioredoxin, catalase, cytochrome-c-oxidase, peroxiredoxin 6, troponin T, CaM kinase II alpha, proteasome subunit N3 and cathepsin L, will be important for understanding the molecular mechanisms underlying pH reduction.
大气中 CO2 分压的增加降低了海洋生态系统中的海水 pH 值，这一过程被称为海洋酸化 (OA)。在幼虫发育的关键阶段，OA 可能对一些海洋物种产生灾难性的后果，包括中国和东南亚商业上重要的海蜗牛 Babylonia areolata (Link 1807)。为了研究 OA 如何影响 Babylonia areolata 的蛋白质组，我们在这里使用无标记蛋白质组学来研究在酸化（pH 7.6）或环境海水（pH 8.1）条件下，B. areolata 三个幼虫发育阶段（未附着的帽贝幼虫 (E1)、附着后的帽贝幼虫 (E2) 和肉食性幼螺 (E3)）中对蛋白质变化的响应。总共鉴定到 720 种蛋白质。这一结果表明酸化严重影响附着后的晚期帽贝幼虫阶段 (E2)。进一步研究差异表达蛋白的作用，包括谷氧还蛋白、热休克蛋白 70、硫氧还蛋白、过氧化氢酶、细胞色素 c-氧化酶、过氧化物酶 6、肌钙蛋白 T、钙调蛋白激酶 II alpha、蛋白酶体亚基 N3 和组织蛋白酶 L，对于理解 pH 值降低的分子机制将是重要的。
