Ge X R, Wang J, Lin S J, Yu N X, Che Y F, Gu Y
Shi Yan Sheng Wu Xue Bao. 1989 Sep;22(3):359-65.
Recently Ge et al reported LAC-122, LAC-210 and LAC-163 McAbs against Human non-small cell lung cancer and LSC McAbs against human lung small cell carcinoma. The immunotoxin, composed of McAb LAC-122 conjugated with Ricin A chain has been reported to have the significant cytotoxic effect in vitro on lung adenocarcinoma cell line SPC-A-1 by Tan et al. The LAC-122 alone has no effect on this target cell in the presence of complement from human, rabbit or guinea pig. The tumor associated antigens of human lung cancer have been recognized for many years, but only few reports deal with the common antigens or common epitopes of the lung cancer. From one fusion, 20 hybrids had been observed, all of these culture supernatants could react with target cell by IF. One of them after 4 th cloning, immunoglobulin isotype of the monoclonal antibody thus far obtained belonged to IgM and named to LC-1. Table 1 showed the results of ABC staining of LC-1 with a variety of tumors, normal adult and fetal tissues. From 12 non-small cell lung cancers, including 7 lung adenocarcinoma, 2 lung squamous carcinoma, 3 lung giant cell carcinoma, only one adenocarcinoma gave negative staining. As for 6 small cell lung cancers, all of them showed the positive reaction. It could be also reacted with 11 other tumors.(ABSTRACT TRUNCATED AT 250 WORDS)
最近，葛等人报道了针对人非小细胞肺癌的LAC - 122、LAC - 210和LAC - 163单克隆抗体，以及针对人肺小细胞癌的LSC单克隆抗体。谭等人报道，由与蓖麻毒素A链偶联的LAC - 122单克隆抗体组成的免疫毒素在体外对肺腺癌细胞系SPC - A - 1具有显著的细胞毒性作用。在存在人、兔或豚鼠补体的情况下，单独的LAC - 122对该靶细胞没有作用。人肺癌的肿瘤相关抗原已被认识多年，但只有少数报道涉及肺癌的共同抗原或共同表位。从一次融合中观察到20个杂交瘤，所有这些培养上清液都能通过免疫荧光法与靶细胞反应。其中一个在第4次克隆后，迄今为止获得的单克隆抗体的免疫球蛋白同种型属于IgM，命名为LC - 1。表1显示了LC - 1对多种肿瘤、正常成人和胎儿组织的ABC染色结果。在12例非小细胞肺癌中，包括7例肺腺癌、2例肺鳞癌、3例肺巨细胞癌，只有1例腺癌染色为阴性。至于6例小细胞肺癌，全部显示阳性反应。它也能与其他11种肿瘤发生反应。（摘要截选至250词）
