Shoji M, Kawamoto S, Sato S, Kamei M, Kato M, Hashizume S, Seki K, Yasumoto K, Nagashima A, Nakahashi H
Morinaga Institute of Biological Science, Yokohama, Japan.
Hum Antibodies Hybridomas. 1994;5(3-4):116-22.
In vitro immunization of normal human peripheral lymphocytes by the co-cultivation with A549 cells (a human lung adenocarcinoma cell line), followed by the hybridization with the partner cell line A4H12, resulted in the efficient generation of hybridomas which produce human monoclonal antibodies of IgG or IgM isotype. Immunohistochemical and immunocytological studies showed that one of those monoclonal antibodies, termed AE6F4, was specifically reactive to lung cancer tissues as well as cancer cells in sputa.
通过与A549细胞（一种人肺腺癌细胞系）共培养对正常人外周淋巴细胞进行体外免疫，随后与伴侣细胞系A4H12杂交，有效地产生了分泌IgG或IgM同种型人单克隆抗体的杂交瘤。免疫组织化学和免疫细胞学研究表明，其中一种单克隆抗体，称为AE6F4，对肺癌组织以及痰液中的癌细胞具有特异性反应。
