Greiner T C, Robinson R A, Maves M D
Department of Pathology, College of Medicine, University of Iowa, Iowa City 52242.
Am J Clin Pathol. 1989 Dec;92(6):711-20. doi: 10.1093/ajcp/92.6.711.
Forty-five patients with adenoid cystic carcinoma (ACC) of salivary glands were retrospectively studied to determine the prognostic use of DNA ploidy analysis compared with clinicopathologic features. Nuclear suspensions were prepared from 37 of these tumors by the Hedley technique on paraffin-embedded material. The DNA content was analyzed by flow cytometry after propidium iodide staining. Thirty-five tumors were diploid and 2 were tetraploid. Mean survival was 117 and 52 months for the diploid and tetraploid patients, respectively. The median S-phase fraction (Spf) of the 35 diploid tumors was 4.45%. Of the 17 diploid patients who died of disease, there were 11 tumors with high Spf (greater than 4.45%) and 6 tumors with a low Spf (P less than 0.05 chi-square test). The presence of more than 30% of a solid pattern in the tumor was strongly associated with poor median survival in Kaplan-Meier survival curve analysis (P less than 0.05 log rank test). Grade, stage, recurrence, and metastases were also found to be important prognostic factors. Because few tumors were nondiploid, these results suggest that S-phase fraction analysis may be a more useful prognostic indicator than ploidy classification.
对45例涎腺腺样囊性癌（ACC）患者进行回顾性研究，以确定与临床病理特征相比，DNA倍体分析在预后评估中的作用。采用Hedley技术从其中37例石蜡包埋肿瘤组织制备核悬液。碘化丙啶染色后，通过流式细胞术分析DNA含量。35例肿瘤为二倍体，2例为四倍体。二倍体和四倍体患者的平均生存期分别为117个月和52个月。35例二倍体肿瘤的中位S期分数（Spf）为4.45%。在17例死于疾病的二倍体患者中，有11例肿瘤的Spf较高（大于4.45%），6例肿瘤的Spf较低（卡方检验，P<0.05）。在Kaplan-Meier生存曲线分析中，肿瘤中实性成分超过30%与较差的中位生存期密切相关（对数秩检验，P<0.05）。分级、分期、复发和转移也被发现是重要的预后因素。由于非二倍体肿瘤较少，这些结果表明S期分数分析可能是比倍体分类更有用的预后指标。
