Zhang Ding-Guo, Zhang Jinling, Mao Lin-Lin, Wu Jin-Xia, Cao Wen-Jia, Zheng Jun-Nian, Pei Dong-Sheng
Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou, 221002, China.
Tumour Biol. 2015 May;36(5):3685-91. doi: 10.1007/s13277-014-3007-5. Epub 2015 Jan 7.
p21-Activated kinase 5 (PAK5) is the last identified member of the PAK family. The PAKs are highly conserved serine/threonine and effector proteins for Cdc42 and Rac and are essential in regulating cell motility and survival. Previous studies have demonstrated that PAK5 played a pivotal role in apoptosis, proliferation, cancer migration, and invasion. However, the biological function of PAK5 in hepatocellular carcinoma, as well as its underlying mechanism, still remains to be fully elucidated. In the present study, we demonstrated that PAK5 markedly inhibited cisplatin-induced apoptosis and promoted cell proliferation in hepatocellular carcinoma cells. Moreover, our results showed that overexpression of PAK5 contributed to cell cycle regulation. In order to elucidate the underlying mechanism of PAK5 on cisplatin-induced apoptosis and cell cycle regulation, we also examined the protein expressions of chk2 and p-chk2. In summary, our study investigated the role of PAK5 in cisplatin-induced cellular processes and provided evidence of its underlying mechanism.
p21激活激酶5（PAK5）是PAK家族中最后被发现的成员。PAK是Cdc42和Rac高度保守的丝氨酸/苏氨酸及效应蛋白，在调节细胞运动和存活中起重要作用。先前的研究表明，PAK5在细胞凋亡、增殖、癌症迁移和侵袭中起关键作用。然而，PAK5在肝细胞癌中的生物学功能及其潜在机制仍有待充分阐明。在本研究中，我们证明PAK5显著抑制顺铂诱导的肝细胞癌细胞凋亡并促进细胞增殖。此外，我们的结果表明PAK5的过表达有助于细胞周期调控。为了阐明PAK5对顺铂诱导的细胞凋亡和细胞周期调控的潜在机制，我们还检测了chk2和p-chk2的蛋白表达。总之，我们的研究调查了PAK5在顺铂诱导的细胞过程中的作用，并提供了其潜在机制的证据。
