Hep G2细胞中的胆固醇和胆汁酸合成。26-羟基胆固醇和7α-羟基胆固醇的代谢作用。
Cholesterol and bile acid synthesis in Hep G2 cells. Metabolic effects of 26- and 7 alpha-hydroxycholesterol.
作者信息
Javitt N B, Budai K
机构信息
Division of Hepatic Diseases, New York University Medical Center, NY 10016.
出版信息
Biochem J. 1989 Sep 15;262(3):989-92. doi: 10.1042/bj2620989.
Abstract
- Using a human hepatoma (Hep G2) cell line that continually synthesizes 3 beta-hydroxy-5-cholenoic acid, lithocholic acid, chenodeoxycholic acid and cholic acid we have determined the metabolism and biological effects of 26-hydroxycholesterol and 7 alpha-hydroxycholesterol. 2. Addition of 26-hydroxycholesterol to the medium (6 microM) downregulated cholesterol and chenodeoxycholic acid synthesis. 3. The predominant metabolite of 26-hydroxycholesterol was 3 beta-hydroxy-5-cholenoic acid. 4. Cholesterol synthesis was not affected by the addition of 7 alpha-hydroxycholesterol (6 and 12 microM). The predominant metabolite of 7 alpha-hydroxycholesterol was chenodeoxycholic acid. 5. In Hep G2 cells 7 alpha-hydroxylation of 26-hydroxycholesterol is not well expressed.
摘要
- 使用一种持续合成3β-羟基-5-胆烯酸、石胆酸、鹅去氧胆酸和胆酸的人肝癌（Hep G2）细胞系，我们确定了26-羟基胆固醇和7α-羟基胆固醇的代谢及生物学效应。2. 向培养基中添加26-羟基胆固醇（6微摩尔）可下调胆固醇和鹅去氧胆酸的合成。3. 26-羟基胆固醇的主要代谢产物是3β-羟基-5-胆烯酸。4. 添加7α-羟基胆固醇（6和12微摩尔）不影响胆固醇的合成。7α-羟基胆固醇的主要代谢产物是鹅去氧胆酸。5. 在Hep G2细胞中，26-羟基胆固醇的7α-羟基化表达不佳。
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